Tetrasodium 3,3'-[[6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diyl]bis[imino(2-methyl-4,1-phenylene)azo]]bisnaphthalene-1,5-disulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Qualifier:

according to guideline

Guideline:

other: UBA test protocol: Letale Wirkung beim Zebrabaerbling Brachydanio rerio, (LC 0, LC 50, LC 100; 48 – 96H) Stand Mai 1984

Version / remarks:

Stand Mai 1984

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: direct weighing

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: West Aquarium GmbH (Germany)

ACCLIMATION
- Acclimation conditions: same as test
- Health during acclimation (any mortality observed): mortadility < 5 %
- Medication; none

FEEDING DURING TEST
- no feeding during the exposure period

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

13 ° dH

Test temperature:

The temperature varied between 20.8 - 21.6 °C (1990-10-09)
The temperature varied between 21.5 - 22.8 °C (1990-10-16)

pH:

pH-values varied betweeen 6.6 - 7.6 (1990-10-09)
pH-values varied betweeen 7.2 - 8.1 (1990-10-16)

Dissolved oxygen:

Oxygen concentration varied between 7.6 - 9.0 mg/L (1990-10-09)
Oxygen concentration varied between 7.6 - 9.0 mg/L (1990-10-16)

Salinity:

no data

Conductivity:

no data

Nominal and measured concentrations:

10, 100, 316 and 1000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria holding 5 L of test media covered by glass plates
- Type (delete if not applicable):closed
- Material, size, headspace, fill volume:
- Aeration: gentle aeration
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Freshwater prepared after ISO: acidity: 0.1 +/- 0.02 mmol/L; Ca / Mg: 4:1; pH: 8.0 – 8.4; oxygen: >= 90 % of saturation.

OTHER TEST CONDITIONS
- Photoperiod: 16 h light: 8 h dark
- The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 0, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed

TEST CONCENTRATIONS
- Range finding study: 10, 100 and 1000 mg/L.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Remarks:

geometric mean LC0/LC100

Effect conc.:

> 526 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Remarks:

mortality in the controls did not exceed 10 %, dissolved oxygen concentration remained above 60 % of the air-saturation value throughout the exposure period, pH did not vary by more than 1 unit

Conclusions:

After 96 hours the following values were determined: LC 0: 316 mg/L, LC 100: 1000 mg/L, Geometric Meen: 562 mg/L.

Executive summary:

In accordance with UBA test protocol: Letale Wirkung beim Zebrabaerbling Brachydanio rerio, (LC 0, LC 50, LC 100; 48 – 96H) Stand Mai 1984 a study was performed to assess the acute toxicity of Bayscript Gelb BR to Danio rerio under static conditions.

Groups of ten fish were exposed to a range of concentrations, nominally 10, 100, 316 and 1000 mg/L of Bayscript Gelb BR dissolved in dilution water. Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 0, 24, 48, 72 and 96 hours of exposure. During the test a temperature range of 20 - 24 °C was maintained in the test vessels, with a maximum temperature fluctuation of +/- 1 °C in the test. The temperature, the pH and the oxygen values were measured at the beginning of the test and every 24 hours thereafter.

After 96 hours the following values were determined: LC 0: 316 mg/L, LC 100: 1000 mg/L, Geometric Meen: 562 mg/L.

Description of key information

After 96 hours the following values were determined: LC 0: 316 mg/L, LC 100: 1000 mg/L, Geometric Meen: 562 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

562 mg/L