Reaction products resulting from the esterification of C18 unsaturated fatty acid with glycerol and glycerol oligomers

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-10-29 to 1992-10-29

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, no GLP

Cross-reference

Reason / purpose for cross-reference:

read-across source

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Di(isooctadecanoic) acid, diester with oxydi(propanediol)

EC Number:

267-821-6

EC Name:

Di(isooctadecanoic) acid, diester with oxydi(propanediol)

Cas Number:

67938-21-0

Molecular formula:

C42H82O7

IUPAC Name:

di(isooctadecanoic) acid, diester with oxydi(propanediol)

Details on test material:

-Lot E 06209496
-Purity ca. 100%
-Hoechst Code 06-FMFW 155

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

none

Test solutions

Vehicle:

no

Details on test solutions:

-Due to low solubility direct weighing into test medium
-Test concentration 500-1000 mg/L

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Activiated sludge concentration: 1600 mg/L dw.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

none

Test conditions

Test temperature:

20 +/- 2 deg C

pH:

7.2 +/- 0.2

Dissolved oxygen:

saturated

Salinity:

none

Nominal and measured concentrations:

nominal

Details on test conditions:

according OECD Guideline 209

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

No effects on the respiration rate was observed at the highest tested concentration of 1000 mg/L.

Results with reference substance (positive control):

Test concentrations with the reference substance: 5-20-30 mg/L. Result within Guideline range.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The registration substance did not show any effect on the respiration rate of the activated municipal sludge at the highest tested concentration of 1000 mg/L.

Executive summary:

In an OECD 209 Guideline study on the inhibition of the respiration rate of municipal activated sludge no effect was found at the highest tested concentration of 1000 mg/L.