Sodium 2-propyne-1-sulphonate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro DNA damage and/or repair study

Remarks:

Type of genotoxicity: DNA damage and/or repair

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

Evaluation of a DNA Polymerase-Deficient Mutant of E. coli for the Rapid Detection of Carcinogens

Author:

Eugene R. Fluck, Lionel A. Poirier And Hans W. Ruelius

Year:

1976

Bibliographic source:

Chem. Biol. Interactions, 15 (1976) 219-231

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

Differential growth inhibition of two E. coli cultures was evaluated as a rapid screening technique for evaluating the mutagenic nature of Methane sulphonic acid

GLP compliance:

not specified

Type of assay:

DNA damage and repair assay, unscheduled DNA synthesis in mammalian cells in vitro

Test material

Test material information

Constituent 1

Reference substance name:

Methanesulphonic acid

EC Number:

200-898-6

EC Name:

Methanesulphonic acid

Cas Number:

75-75-2

IUPAC Name:

methanesulfonic acid

Constituent 2

Reference substance name:

Methane sulphonic acid

IUPAC Name:

Methane sulphonic acid

Details on test material:

- Name of test material: Methane sulphonic acid
- Molecular formula: CH4O3S
- Molecular weight: 96.1056 g/mol
- Substance type: Organic
- Physical state: No data available
- Purity: > 99 % w/w
- Impurities (identity and concentrations): No data available

Method

Target gene:

No data available

Species / strain

Species / strain / cell type:

E. coli, other: Parent strain: P3110 DNA polymerase deficient strain: P3478

Details on mammalian cell type (if applicable):

No data available

Additional strain / cell type characteristics:

other: Pol A+ and pol A- strains

Metabolic activation:

without

Metabolic activation system:

S9 extract was prepared from the livers of male Charles River rats.

Test concentrations with justification for top dose:

25 µL/plate (37 µg/plate )

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: No data available
- Justification for choice of solvent/vehicle: No data available

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: Dimethyl sulfate, ampicillin and colistin

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar

DURATION
- Preincubation period: No data available
- Exposure duration: No data available
- Expression time (cells in growth medium): 16hrs
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available

SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available

NUMBER OF REPLICATIONS: 2

NUMBER OF CELLS EVALUATED: No data available

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available

OTHER EXAMINATIONS: No data available
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available

OTHER:

Evaluation criteria:

Zone of inhibition less than 4mm was considered non significant.

60 mm zone of inhibition was the maximum zone of inhibition amenable to quantitative interpretation.

Statistics:

No data available

Results and discussion

Test results

Species / strain:

E. coli, other:

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

No data available

Remarks on result:

other: strain/cell type:

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative without metabolic activation

Methane sulphonic acid did not cause growth inhibition due to DNA damage in two strains of Escherichia coli exposed to 25 µL/plate without S9 metabolic activation and hence is negative foe gene mutation in vitro.

Executive summary:

Differential growth inhibition of twoE. colicultures (DNA polymerase deficient strain P3478 and parent strain P3110) was evaluated as a rapid screening technique for evaluating the mutagenic nature of Methane sulphonic acid.

 

In a typical assay, Methane sulphonic acidwas applied to two plates containing the pol A⁺organism and two plates containing the pol A^-organism. The plates were then incubated for 16 hrs and the zones of inhibition were measured.

 

Methane sulphonic acid did not cause growth inhibition due to DNA damage in two strains ofEscherichia coliexposed to 50 µL/plate without S9 metabolic activation and hence is negative foe gene mutation in vitro.