Ammonium 4-acetoacetylamino-5-methoxy-2-methylbenzenesulphonate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well performed OECD study wihout GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ammonium 4-acetoacetylamino-5-methoxy-2-methylbenzenesulphonate

EC Number:

276-769-3

EC Name:

Ammonium 4-acetoacetylamino-5-methoxy-2-methylbenzenesulphonate

Cas Number:

72705-22-7

Molecular formula:

C12H15NO6S.H3N

IUPAC Name:

ammonium 4-acetoacetylamino-5-methoxy-2-methylbenzenesulphonate

Details on test material:

purity: 96.3 %

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial, non-adapted

Details on inoculum:

Mixture of activated sludge from the sewage treatment plant of the Hoechst AG at Frankfurt am Main, Höchst with activated sludge from the sewage treatment plant at Hoechst AG, former site Kelsterbach in a ratio of 30 : 70.

Preparation of the suspension:
Washing the sediment from activated sewage sludge two times by centrifuging and re-suspending in mineral medium

Amount of inoculum used : 12 g/L of wet centrifuged sludge were filled into the batch

Content of suspended solids : 0.1121 mg dry mass/g of wet sludge corresponding to 1345 mg dry mass /L in the batches

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

443 mg/L

Based on:

DOC

Initial test substance concentration 2

Initial conc.:

561 mg/L

Based on:

COD

Initial test substance concentration 3

Initial conc.:

630 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

Reaction vessel : 3-L glass beakers with magnetic stirrers, through which air was bubbled. The final volume of the batch was 2000 ml

Reference substance

Reference substance:

diethylene glycol

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

3

Sampling time:

1 d

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

8 d

% Degradation 3

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

17 d

% Degradation 4

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

1 day 26% degradation
3 days 47% degradation
6 days 100% degradation
8 days 93% degradation

Any other information on results incl. tables

A Zahn-Wellens Test according to OECD guideline 302B was performed to determine the inherent biodegradability of the substance.

No degradation could be observed.

The degradation of the reference substance was in the demanded range and showed a high activity of the inoculum. It caused a DOC-reduction of more than 90 % within only 7 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The results of testing the biodegradability in the Zahn-Wellens Test revealed that:
Acetoacet-cresidine-sulfonic acid NH4-salt was not biodegradable under the conditions of this test.