DL-serinohydrazide monohydrochloride

Melting point / freezing point

Administrative data

Endpoint:

melting point/freezing point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01 October 2014-08 January 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-compliant study in accordance with EC A.1, OECD 102 and EPA OPPTS 830.7200.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method A.1 (Melting / Freezing Temperature)

Version / remarks:

(2008)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 102 (Melting point / Melting Range)

Version / remarks:

(1995)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7200 (Melting Point / Melting Range)

Version / remarks:

(1998)

Deviations:

no

GLP compliance:

yes

Type of method:

thermal analysis

Remarks:

(differential scanning calorimetry)

Test material

Test material information

Constituent 1

Reference substance name:

DL-serinohydrazide monohydrochloride

EC Number:

259-839-8

EC Name:

DL-serinohydrazide monohydrochloride

Cas Number:

55819-71-1

Molecular formula:

C3H9N3O2.ClH

IUPAC Name:

2-amino-3-hydroxy-propanehydrazide;hydrochloride

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): DL Serine hydrazide
- Molecular formula: C3H9N3O2.HCl
- Molecular weight: 155.6 g/mol
- Description: White powder
- Storage condition of test material: At room temperature in the dark under nitrogen
- Expiration date of the lot/batch: 22 April 2016

Results and discussion

Melting / freezing point

Melting / freezing pt.:

198 °C

Decomposition:

yes

Decomp. temp.:

>= 198 °C

Remarks on result:

other: Mean of 197.94°C and 197.99°C. Melt. pt in K: 471. Decomposition during and directly after melting.

Any other information on results incl. tables

PRELIMINARY TEST WITH TGA

From 200°C upwards the weight of the sample decreased significantly. At 228°C, the sample weight had decreased by 25%. After the experiment, black residue was observed on the lid of the sample container and a black expanded residue remained in the sample container.

 

DSC EXPERIMENTS

Experiment 1: An endothermic peak was observed between 175°C and 228°C followed by an exothermic effect. The endothermic effect was most likely obtained due to melting of the test substance. The extrapolated onset temperature of the peak was 197.94°C. The exothermic effect were related to reaction and/or decomposition of the test substance. After the experiment, black residue was observed on the lid of the sample container and a black expanded residue remained in the sample container.

 

Experiment 2: To investigate the melting peak the temperature program was stopped during the endothermic effect. The extrapolated onset temperature of the melting peak was 197.99°C. After the experiment, a dark brown molten residue remained in the sample container. The results indicated that the test substance reacts and/or decomposes during melting. 

Applicant's summary and conclusion

Conclusions:

In a GLP-compliant study in accordance with EC A.1, OECD 102 and EPA OPPTS 830.7200 using differential scanning calorimetry, the melting temperature of the substance has been determined as 198°C. Reaction and/or decomposition of the test substance was observed during and directly after melting.