Reaction products of triphenyl phosphite and isodecanol (1:2)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: The reliability of this study is in question because of the likely potential that daphnia were exposed to emulsions/free product given that concentrations were run above the water solubility limit.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 84/449/EEC C.2, Acute toxicity for Daphnia [Comparable to OECD 202]

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of triphenyl phosphite and isodecanol (1:2)

EC Number:

701-374-4

Cas Number:

25550-98-5

Molecular formula:

C26H47O3P

IUPAC Name:

Reaction products of triphenyl phosphite and isodecanol (1:2)

Details on test material:

- Name of test substance: Irgastab CH 300 (CAS# 25550-98-5)- Commercial, purity: Stated to be ‘within specifications’- Commercial batch no. 09520538 (Test identification code: TK 11211) - Supplier: Ciba-Geigy, Ltd, Basel, Switzerland

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

The test substance stock solution was test substance (20.1 mg) mixed with water and made up to 1000 mL. Calculated amounts of the stock solution to produce the desired test concentrations were added to the water and were homogeneously distributed.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name:
- Strain: Straus 1820
- Source: Ciba-Giegy ecotoxicology testing facility
- Age at study initiation (mean and range, SD):
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Feeding during test: At each water renewal the Daphnia were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract.


ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):


QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

240 mg/CaCO3/L

Test temperature:

20 +/- 1oC

pH:

The pH values of the water ranged from 8.0 to 8.6 at 0 hours and 7.8 to 7.9 at 48 hours.

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

The nominal test concentrations were 0 (blank control), 0.058, 0.1, 0.18, 0.32, 0.58, and 1.0 mg of test substance/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass vessels
- Type: covered with watch glasses
- Material, size, headspace, fill volume: 100 mL of solution.
- Aeration: aerated with clean air for at least 24 hours prior to use
- Renewal rate of test solution/water: water partially renewed three times per week
- No. of organisms per vessel: There were a total number of 20 Daphnia per treatment group/4 replicates of 5 Daphnia each.
- No. of vessels per concentration (replicates): 4 replicates of 5 Daphnia each.
- No. of vessels per control (replicates): 4 replicates of 5 Daphnia each.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours/day
- Light intensity: fluorescent lighting

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.6 mg/L

Basis for effect:

mobility

Remarks on result:

other: 0.4-1.2 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.2 mg/L

Basis for effect:

mobility

Remarks on result:

other: 0.1-0.4 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

< 0.04 µg/L

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 0.83 mg/L

Basis for effect:

mobility

Effect concentrations 5

Dose descriptor:

NOEC

Effect conc.:

< 0.04 mg/L

Basis for effect:

mobility

Effect concentrations 6

Dose descriptor:

LOEC

Effect conc.:

0.04 mg/L

Basis for effect:

mobility

Details on results:

Immobilization was recorded after 24 and 48 hours of exposure. The EC-50 values were calculated according to method described by Berkson (1953; JASA 48:569-599), and the EC-values were graphically determined on gausso-logarithmic probability paper.

Any other information on results incl. tables

The following are the percent of the immoblizedDaphnia(n = 20 per test concentration) after 24 and 48 hours for each test group:

                                                                                                              24 Hours                  48 Hours

                                                           Test Concentration Total No. (%)                       Total No. (%)

                                                           Blank                              0 (0)                          0 (0)

                                                           0.04 mg/L                      1 (5)                          5 (25)

                                                           0.17 mg/L                      1 (5)                          8 (40)

                                                           0.38 mg/L                      2 (10)                       7 (35)

                                                           0.45 mg/L                      9 (45)                      18 (90)

                                                           0.83 mg/L                     15 (75)                     19 (95)

 

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Conclusions:

The 48-hr EC50 value was determined to be 0.2 mg/L based on immobility, though test results cannot be used reliably.

Executive summary:

The 48-hr EC50 value was determined to be 0.2 mg/L based on immobility, though test results cannot be used reliably.