Reaction products of triphenyl phosphite and isodecanol (1:2)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Comparable to OECD 203 with the exception of a 3-day fast prior to exposure rather than a fast of 24-hours. The exposure duration was 48-hours, not 96-hours as preferred per the study guideline. Study was run at a concentration well in excess of the water solubility limit.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of triphenyl phosphite and isodecanol (1:2)

EC Number:

701-374-4

Cas Number:

25550-98-5

Molecular formula:

C26H47O3P

IUPAC Name:

Reaction products of triphenyl phosphite and isodecanol (1:2)

Details on test material:

- Name of test substance: Irgastab CH 300 (CAS# 25550-98-5)
- Commercial purity: Not stated
- Lot/batch No.: 928 (Test article identification code: TK 11211)
- Supplier: Ciba-Geigy, Ltd, Basel, Switzerland

Test solutions

Vehicle:

yes

Details on test solutions:

The test substance was prepared by dissolving 5.0 g into 50 mL of DMSO. No analytical calculations of test concentrations were performed, but the material appeared to be homogeneously distributed.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

- Common name: Golden Orfe
- Source: P. Hohler, Zeiningen, Switzerland
- Length at study initiation: 44 mm (35-50 mm)
- Weight at study initiation: 0.59 g (0.29-0.85g).
- Feeding during test: None

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

254 mg CaCO3/L

Test temperature:

21 +/- 1oC.

pH:

7.8 to 8.1

Dissolved oxygen:

97-98 % at 0 hours, 84-94% at 24 hours, and 93-99% at 48 hours

Nominal and measured concentrations:

The highest concentration of test substance in water was determined to be 100 mg/L, based on visible precipitate immediately forming at concentrations above this value. (Nominal concentration)

Details on test conditions:

Calculated amounts of the test substance to produce the desired test concentrations were added into the water and were homogeneously distributed. The fish were transferred into the tank for a total duration of 48 hours.

TEST SYSTEM
- Test vessel: 20-L tank
- Aeration: Gentle aeration during the test
- No. of organisms per vessel: Ten fish
- No. of vessels per concentration (replicates): Duplicate test article groups were used and compared to that of concurrent blank (water) and vehicle control
- Biomass loading rate: 0.39 g fish/L water.

OTHER TEST CONDITIONS
- Photoperiod: 16-hours daily were used.
- Light intensity: fluorescent light

EFFECT PARAMETERS MEASURED: Signs and symptoms of toxicity and mortality were recorded at 24 and 48 hours.

VEHICLE CONTROL PERFORMED: yes, 1101 mg DMSO/L water

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: no effects

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

No effects were observed up to a maximum loading level of 100 mg/L. This study was run at concentrations well above the water solubility limit; however, since there are no signs of toxicity even at such a high concentration the study is acceptable to establishing that this phosphite is not toxic to fish.

Executive summary:

No effects were observed up to a maximum loading level of 100 mg/L (above the estimated solubility limit).