Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-137-0 | CAS number: 78-73-9
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
RA_CAS 67-48-1_Pseudomonas putida_DIN 38412 Part 8: EC10(17h): 112.90 mg/L, EC50(17h): 132.80 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 132.8 mg/L
- EC10 or NOEC for microorganisms:
- 112.9 mg/L
Additional information
Toxicity to aquatic algae was not investigated with the target substance Choline hydrogen carbonate. A valid experimental result is available for the reliable read-across substance Choline chloride (CAS 67-48-1). Both substances belong to the group of quaternary amine salts (more specific: choline salts, i.e. (2-hydroxyethyl)trimethylammonium salts), which will dissociate immediately in aqueous environment. Choline and its metabolites are important for different functions in the body of animate beings and thus a naturally occurring compound. Thus, the substance characteristics of both substances are comparable. For the detailed justification of this procedure, please refer to the separate read-across statement by Chemservice S.A. (2013).
The toxicity of Choline chloride (dissolved in water) towards Pseudomonas putida was investigated according to the German guideline DIN 38412 Part 8 (BASF AG, 2003). The experiment was conducted at 297 +/- 1 K (22.85 - 24.85 °C) for a time duration of 17 h. The test concentrations were 0 (control), 50, 100, 150, 200, 250 and 300 mg/L. The following results were obtained: EC10(17h): 112.90 mg/L, EC50(17h): 132.80 mg/L and EC90(17h): 278.20 mg/L, respectively. Hence, Choline chloride is non-toxic to Pseudomonas putida.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
