Registration Dossier
Registration Dossier
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EC number: 939-039-1 | CAS number: -
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
There is no statistically difference between Control and the treatment 100 mg/L. As a consequence, it can be considered that the test item has no effect Daphnia magna reproduction.
Key value for chemical safety assessment
Additional information
A 21-day Daphnia magna reproduction study was conducted to determine the effects of exposure to the test item on Daphnia magna reproduction and survival in accordance with the OECD Chemicals Testing Guideline No.211 Daphnia magna Reproduction Test (adopted October 2012). Based on the information supplied by the Sponsor, the substance has no acute effect on daphnids (up to the highest concentration, i.e. 1000 mg/L). Therefore, the study was conducted as a semi-static limit test (at nominal concentrations of 100 mg/L) with renewal of test media three times a week during the test. A control group was also included. Ten replicate test vessels were prepared for the control and the test concentration. A single juvenile Daphnia magna (<24 hours old selected by filtration) was added to each test vessel. On renewal occasions as required, parental animals were transferred into freshly prepared test media. Any juveniles remaining in old test media were counted and the number of juveniles produced per parental animal on any day during the 21-day study duration was recorded. There was 10% mortality of the parental Daphnia magna in the control and 30% mortality in the 100 mg/L test group. No chemical analysis in support of the definitive test was conducted due to the high insolubility of this UVCB substance, which does not allow the measurement of the concentration of all the UVCB in the medium. The total number of juveniles produced by surviving adults by Day 21 was tested for normality using the Shapiro Wilks test and was found to be normally distributed. Homogeneity of variance between the two tested group was confirmed by the Student-t-Test: there is no statistically difference between Control and the treatment 100 mg/L. As a consequence, it can be considered that the test item has no effect on Daphnia magna reproduction.
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