Sodium 2-amino-4,6-dinitrophenoxide

Endpoint summary

Description of key information

Short term toxicity to fish:

Based on the piction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to fish was predicted. On the basis of this program, the LC50 value for short term toxicity to aquatic invertebrates was predicted to be1920.657mg/l for test material in 48 hrs. Based on the above effect concentration value it can be predicted test substance is not toxic to fish and can not be classified as per CLP criteria.

Short term toxicity to aquatic invertebrates:

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 1637.454 mg/L. Thus considering the value from CLP Criteria for aquatic classification of the substance , it is concluded that test material does not exhibit short term toxicity aquatic invertebrate (Daphnia Magna) in the present test condition.

Toxicity of aquatic algae and cyanobacteria:

The short-term toxicity of the test substance to green algae is predicted using EPI Suite ECOSAR version 1.10. On the basis of effects observed in a static freshwater system during a 96 hr exposure, the effect concentration (EC50) for the substance is estimated to be461.852 mg/L. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.

 

Toxicity to microorganism:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical .The studies are as mentioned below:

Acute toxicity of test material was evaluated for microorganisms, the test material showed no effect on Pseudomonas putida when tested for 16 h at concentration 7 mg/l.

In another study , the effect of test material was evaluated on microorganism Photobacterium phosphoreum for 30 min inhibition of bioluminance test  the EC50 was obseved to be 29 mg/l. The test chemical  is  likely to be toxic to microorganism atleast in the concentration  range of 7 - 29 mg/l

Additional information

Short term toxicity to fish:

The toxicity of test material was evaluated for fish based on the predicted data and data for structurally similar read across substance from peer reviewed journal

Based on the piction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to fish was predicted. On the basis of this program, the LC50 value for short term toxicity to aquatic invertebrates was predicted to be1920.657mg/l for test material in 48 hrs. Based on the above effect concentration value it can be predicted test substance is not toxic to fish and can not be classified as per CLP criteria.

The short term toxicity of test material on fish Oncorhynchus mykiss (Rainbow trout) was observed for 96 h Juvenile rainbow trout (x = 41.6±8.7 mm total length) were selected .Rainbow trout were held in the laboratory at an approximate density o 3 g/l in continuous flow, non - chlorinated deep well water with 99 percent replacement of the water every 12 h. The fish were acclimatized for 2 weeks in the same conditions as test ,during acclimation, the trout were fed commercial trout chow daily, static renewations were conducted. In an effort to control possible volatilization, the aquaria were fitted with glass covers. Test chambers were placed in two 4 x 0.8 x 0.25 m circulated constant temperature baths. The dilution water used during the tests was the same as that used for acclimation.Two replicates composed of a total of 25 fish (N = 13 and 12) were randomly selected from the test population and placed in the aquaria containing the test solutions. The nominal test concentrations for test material were 0, 75, 85, 95, 105, 115, and 125 mg/l. The lethal effect concentration (LC50) observed after 96 h was noted to be 135.0 mg/l. As no mortalities were observed till the concentration 135.0 mg/l , it can be concluded that the test material is not classified as per CLP criteria.

For another structurally similar read across substance, the short term toxicity of test material on fish Oncorhynchus mykiss (Rainbow trout) was observed for 96 h Juvenile rainbow trout (x = 41.6±8.7 mm total length) were selected .Rainbow trout were held in the laboratory at an approximate density o 3 g/l in continuous flow, non - chlorinated deep well water with 99 percent replacement of the water every 12 h. The fish were acclimatized for 2 weeks in the same conditions as test ,during acclimation, the trout were fed commercial trout chow daily, static renewations were conducted. In an effort to control possible volatilization, the aquaria were fitted with glass covers. Test chambers were placed in two 4 x 0.8 x 0.25 m circulated constant temperature baths. The dilution water used during the tests was the same as that used for acclimation.Two replicates composed of a total of 25 fish (N = 13 and 12) were randomly selected from the test population and placed in the aquaria containing the test solutions. The nominal test concentrations for test material were 0, 75, 85, 95, 105, 115, and 125 mg/l. The lethal effect concentration (LC50) observed after 96 h was noted to be 135.0 mg/l.

As no mortalities were observed till the concentration 219.2 mg/l , it can be concluded that the test material is not classified as per CLP criteria.

Short term toxicity to aquatic invertebrates:

The toxicity of test material was evaluated for aquatic invertebrate based on the predicted data and data for structurally similar read across substance from peer reviewed journal.

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 1637.454 mg/L. Thus considering the value from CLP Criteria for aquatic classification of the substance , it is concluded that test material does not exhibit short term toxicity aquatic invertebrate (Daphnia Magna) in the present test condition.

The above prediction was supported by data from peer reviewed journal for structurally similar read across substance ,Short term toxicity of test material was evaluated on aquatic invertebrate , for 24 h .The dilution water used had a specific composition: acid capacity Ks4.3 of 0.8mmoll -I total hardness of 2.4 mmol/l , a calcium to magnesium ratio of 4:1, a sodium to potassium ratio of 10:1 and thus a resulting initial pH value of 8.0 + 0.2. 6-24 h old daphnids were used , test was carried out in 50 ml beakers with ground-glass stoppers each with 20 ml useful capacity. The effect concentration noted after 24 h was observed to be 123 mg/l. Based on the above effect concentration it can be considered that test material is not toxic to daphnia magna and hence, can not be classified as per CLP criteria.

Another study , for structurally similar read across substance stated that toxicity of test material was evlauted on American Oysters . Clutchless American oysters (x = 48.4 ± 6.2 mm shell height) were purchased from the Wilde Oyster Hatchery, Shady Side, Maryland.test organisms were obtained at approximately 10 C and acclimated in the laboratory for a minimum of two weeks at 10 (+ 1) C under a 12 hlight:12 h dark photoperiod prior to and during testing. Oysters were fed natural plankton in the estuarine water system supplemented with 1.5 percent corn starch-yeast solution when necessary.One replicate of 20 organisms was used at each test concentration , the concentrations used were 0, 25, 50, 100, 150, and 200 mg/l. The effect of test material was on organism American Oysters for 144 h , was observed to be 254.9 mg/l.

Based on the above effect concentration it can be concluded that test material is not toxic to aquatic invertebrates.

Toxicity of aquatic algae and cyanobacteria:

The toxicity of test material was evaluated for aquatic algae and cyanobacteria based on the predicted data and data for structurally similar read across substance from peer reviewed journal.

The short-term toxicity of the test substance to green algae is predicted using EPI Suite ECOSAR version 1.10. On the basis of effects observed in a static freshwater system during a 96 hr exposure, the effect concentration (EC50) for the substance is estimated to be461.852 mg/L. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.

 In another study ,The Scenedesmus cell multiplication inhibition test was performed .The cultivation of the preliminary cultures was undertaken 3 days prior to the preparation of the test solution. For this purpose, 50 ml nutrient solution were filled into 300 ml Erlenmeyer flasks with metal caps and inoculated with Scenedesmus from 7-day-old stock cultures. The cell concentration in the preliminary culture flasks must amount to 10⁴ml-L . The effect concentration EC50 observed after 96 h was noted to be >500 mg/l.

Based on the above effect concentration it can be concluded that test material is not clasiffied for aquatic algae and cyanobacteria.

Toxicity to microorganism:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical .The studies are as mentioned below:

Acute toxicity of test material was evaluated for microorganisms, the test material showed no effect on Pseudomonas putida when tested for 16 h at concentration 7 mg/l.

In another study , the effect of test material was evaluated on microorganism Photobacterium phosphoreum for 30 min inhibition of bioluminance test  the EC50 was obseved to be 29 mg/l. The test chemical  is  likely to be toxic to microorganism atleast in the concentration  range of 7 - 29 mg/l