Registration Dossier
Registration Dossier
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EC number: 425-150-8 | CAS number: 94723-86-1
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 425-150-8
- EC Name:
- -
- Cas Number:
- 94723-86-1
- Molecular formula:
- C15 H22 O3 S
- IUPAC Name:
- 2-butanoyl-3-hydroxy-5-(thian-3-yl)cyclohex-2-en-1-one
- Details on test material:
- - Name of test material (as cited in study report): 625-Triketon
- Physical state: solid, beige
- Analytical purity: 94%
- Batch No.: LJ 27881/1
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: was added directly
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: Municipal activated sludge taken from a laboratory wastewater treatment plant fed with municipal sewage.
- Initial biomass concentration: 1g/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
Test conditions
- Test temperature:
- 20 ± 2 °C
- Nominal and measured concentrations:
- Nominal test concentration: 20, 100, 250, 500, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml fill volume, 8 ml/ vessel synthetic medium (OECD medium)
- No. of vessels per concentration (replicates): 1
- Biomass loading rate: 1g /L - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 630 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- Effect concentrations (30 min):
EC20 = < 1mg/L
EC50 = 10 mg/L
EC80 = ca. 62 mg/L
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
