Androst-5-ene-17-carbonitrile, 3,3-[1,2-ethanediylbis(oxy)]-17-hydroxy-, (17.alpha.)-

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Mar 1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to DIN guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,3-Ethylenedioxy-17 alpha-hydroxy-5-androstene-17 beta-carbonitrile

EC Number:

617-449-9

Cas Number:

83196-58-1

Molecular formula:

C22 H31 N O3

IUPAC Name:

3,3-Ethylenedioxy-17 alpha-hydroxy-5-androstene-17 beta-carbonitrile

Details on test material:

- Name of test material (as cited in study report):
- Analytical purity: 90.6%
- Lot/batch No.: 26025160

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pseudomonas putida

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

0.66 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 2

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

0.84 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Any other information on results incl. tables

EC90 = 1.0 mg/L

Table 1: Turbidity of the test and control solutions (TE/F)

Concentration		0 hour				16 hours
(mg/I)	Test vessels	1	2	3	1	2	3
0		0	0	0	620	684	544
0,34		-	-	-	636	616	630
0,68		-	-	-	614	624	596
1,36		-	-	-	546	560	514
2,72		-	-	-	522	560	542

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The results of the test with Pseudomonas putida clearly demonstrate that androstendionecyanhydrinethylene
ketal inhibits the growth of the test organisms at concentrations which are
lower than the maximum solubility of the test compound.

Executive summary:

The test substance androstendione-cyanhydrinethylene ketal was incubated in an aqueous solution including nutrients, with a bacterial population containing Pseudomonas putida for a test duration of approx. 16 hours. The nutrient solution was made up of mainly nitrate, phosphates, carbon hydrate, some trace elements and vitamins. The test concentrations were: 0.34; 0.68; 1.36 and 2.72 mg/l (nominal). Additionally, one control vessel without the test substance was used. All test concentrations including the control were incubated in tripie determinations. Furthermore, for each test concentrationone test vessel was incubated without addition of the inoculum, in order to analyze the inherent turbidity of the test compound. As a parameter for the growth of the bacterial population, the turbidity of the test and control solutions was analyzed photometrically at a wave-Iength of 436 nm. The extinction units were calculated in TE/F units.

The values of the turbidity measurements clearly demonstrate that inhibitory effects on the growth of the bacterial population were induced by androstendione-cyanhydrinethylene ketal. The EC50 of androstendione-cyanhydrinethylene ketal is 0.82 mg/I, which is lower than the maximum solubility of the test compound under the conditions of the test, which was determined to be 3.4 mg/I.