Benzenesulfonic acid, 2-amino-4,5-dichloro-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412 part L15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-4,5-dichlorobenzene-1-sulfonic acid

EC Number:

700-413-2

Cas Number:

6331-96-0

Molecular formula:

C6 H5 Cl2 N O3 S

IUPAC Name:

2-amino-4,5-dichlorobenzene-1-sulfonic acid

Details on test material:

- Name of test material (as cited in study report): Dichlororthanilsaeure (trocken)
- Lot/batch No.: P 539/88
- Analytical purity: 98.8 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The product was added to the test water without any pretreatment
- Evidence of undissolved material (e.g. precipitate, surface film, etc): increasing amounts of undissolved test substance accumulated at the water surface and at the bottom of the aquaria with increasing test concetrations. After pH adustment at a concentration of 1000 m/l the test substance was completely dissolved.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Eggers, Hohenwestedt, Germany.
- Length at study initiation (length definition, mean, range and SD): 7.0 cm (range: 6.5 - 7.8 cm)
- Weight at study initiation (mean and range, SD): 3.4 g (range: 2.5 - 5.1 g)

ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: Growing feed F/B 50, Ssniff Spezialdiäten GmbH, Soest, Germany; ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

23-24 °C

pH:

after 1 h:
7.5 (100 mg/l)
3.0 (215 mg/l)
2.4 (464 mg/l)
2.0 (1000 mg/l)
6.1 (1000 mg/l after pH-adjustment)

Dissolved oxygen:

5.1 - 7.9 mg/l

Nominal and measured concentrations:

Nominal test substance concentrations:
control, 100, 215, 464, 1000 mg/l and 1000 mg/l with pH adjusment

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium (30xX 22x24 cm)
- Fill volume: 10 l
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 3.4 g/l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted freshwater according to DIN 38 412, part 11

OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: 16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 hours


TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: LC50 after 96 hours: 100-1000 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

100 - 220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was observed in the 1000 mg/l group after pH-adjustment.
Apathy was observed at a concentration of 215 mg/l after 4 hours of exposure.

Reported statistics and error estimates:

Finney DJ (1971) Probit Analytics. Cambridge University Press, 3rd ED