Distilled acetalization product between glucose and C12-18(even numbered)alcohol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006-11-20 to 2006-11-24

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This study has been conduced in accordance with an adopted guideline and in compliance with the Good Laboratory Practice.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

2007-01-12

Test material

Test material information

Constituent 1

Reference substance name:

montanov S

IUPAC Name:

montanov S

Details on test material:

Batch: T62211
Appearence: pearls of wax
Purity: ES>99%
Stability: water 96h
Solubility water<0.1g/L
Production date 06.06.08
Expiry date: 07.12.08
Storage: in a closed vessel, at room temperature 20 +/- 5°C

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

As the solubility lies below 100mg/L, the water accomodated fraction was prepared for the test. This was done by weighing of each nominal load, adding the corresponding amount of deionised water and shaking vigorously for 24 hours. The resulting solutions were filtrated through 0.45 um filters.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Species: desmodesmus subspicatus
Strain: Chodat
Family: Chlorophyceae
Order: Chlorococcales
Origin: Institut für Pflanzenphysiologie of Universität Göttingen.
The alga are kept as stock culture on solid agar at 8°C. From an aliquot of this stock culture, the preculture was prepared.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

72 hours

Test conditions

Test temperature:

23-25°C

pH:

8.1-10.1

Nominal and measured concentrations:

0 / 1 /10 /100 mg/L

Details on test conditions:

Vessels: roud cuvettes d =6mm
Duration: 72 h
Lighting: 4440-8880 Lux
Control: deionised water with nutrient medium and algae
Treatments: test solution with nutrient medium and algae

Date: November: 21st - 24th 2006
Treatments tested: 1 / 10 / 100 mg/L
Number of replicates: six replicates for the control, three replicates for each treatment

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 4

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 5

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 6

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 7

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: growth rate, cell number and biomass

Results with reference substance (positive control):

NOEC 72h= 0.1 mg/L
LOEC 72h= 0.16 mg/L
72h ErC50= 1.1 mg/L
72h EbC50= 0.36 mg/L
72h EyC50= 0.30 mg/L
72h ErC100> 1.6 mg/L
72h EbC100> 1.6 mg/L
72h EyC100> 1.6 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

72h NOEC (growth rate)= 100 mg/L
72h NOEC (AUC)= 100 mg/L
72h NOEC (yield)= 100 mg/L

72h LOEC (growth rate) > 100 mg/L
72h LOEC (AUC)> 100 mg/L
72h LOEC (yield)> 100 mg/L

72H EC50> 100 mg/L

Executive summary:

Determinationof the 72h-EC50 of Montanov S against Desmodesmus subspicatus according to OECD 201 resp. EU C.3

As the solubility lies below 100mg/L, the water accomodated fraction was prepared for the test. This was done by weighing of each nominal load, adding the corresponding amount of deionised water and shaking vigorously for 24 hours. The resulting solutions were filtrated through 0.45 um filters.

In the main study, three concentrations of the test item in a geometric series (spaced by factor 10, the highest being 100 mg/L) were used. the treatments were used to incubate the unicellular freshwater green alga Desmodesmus subspicatus for a period of 72 hours. The cell concentration of each replicate was determined by measuring the absorption of the cuvettes at 440 nm every 24 hours with a spectral photometer. With these measured values, the number of cells was calculated (linear correlation between cell concentration and absorption given). Then the growth rate, the area under the growth curve (AUC) and the yield could be determined.

The EC50 of dichromate potassium was tested in a current reference test. The values lay within the normal range of the laboratory.

The following results for the test item Montanov S were determined:

72h NOEC (growth rate)= 100 mg/L

72h NOEC (AUC)= 100 mg/L

72h NOEC (yield)= 100 mg/L

72h LOEC (growth rate) > 100 mg/L

72h LOEC (AUC)> 100 mg/L

72h LOEC (yield)> 100 mg/L

72H EC50> 100 mg/L