Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2011
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report Date:
2011

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic (adaptation not specified)
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
20 mg/L
Based on:
DOC

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
31
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
no
Interpretation of results:
other: not readily biodegradable
Conclusions:
The ready biodegradability of GR-50-0091 was determined by the manometric respirometry test. The test substance undergoes 31% biodegradation after 28d (32% after 34d) in the test conditions.
The curves obtained with the reference substance alone and with GR-50-0091 + reference substance show no toxic effect of GR-50-0091 to the microorganisms at the test concentration (20mg/L).
Thus GR-50-0091 should be regarded as not readily biodegradable according to this test.
Acompanying quantitative analysis for the test substance evidences rapid primary biodegradation of the test substance. Assuming that the primary biodegradation follows a first order kinetic DT50 and DT90 were calculated for 4-vinylcyclohex-1-enecarbaldehyde and 1-vinylcyclohex-3-enecarbaldehyde.
The results were:
DT50(4-vinylcyclohex-1-enecarbaldehyde )=3.5h
DT90(4-vinylcyclohex-1-enecarbaldehyde )=11.7h
DT50(1-vinylcyclohex-3-enecarbaldehyde)=7.4h
DT90(1-vinylcyclohex-3-enecarbaldehyde)=24.6h