Ethyl 5-oxo-1-phenyl-2-pyrazoline-3-carboxylate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well performed study with GLP, guideline not available in this year

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: procedure of Ames and coworkers

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl 5-oxo-1-phenyl-2-pyrazoline-3-carboxylate

EC Number:

201-899-4

EC Name:

Ethyl 5-oxo-1-phenyl-2-pyrazoline-3-carboxylate

Cas Number:

89-33-8

Molecular formula:

C12H12N2O3

IUPAC Name:

ethyl 5-oxo-1-phenyl-4,5-dihydro-1H-pyrazole-3-carboxylate

Method

Species / strain

Species / strain / cell type:

other: S. typhimurium TA 1535, TA 1537, TA1538, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Metabolic activation system:

rat S9-mix

Test concentrations with justification for top dose:

4, 20, 100, 500, 2500, 5000 µg/plate

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: Methylhydrazone Derivative, 2-Aminoanthracene, Streptocotocine, ENNG

Evaluation criteria:

A chemical is considered to have a positive response if the number of induced revertants is more than double the spontaneous mutations.

Statistics:

not required

Results and discussion

Test results

Species / strain:

other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not determined

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

The substance was not mutagenic in the Ames Test.

Executive summary:

The susbatnce was trested for mutagenicity in an Ames Test. Five strains of Salmonella typhimurium, auxotrophic for histidine, and one of Escherichia coli, auxotrophic for tryptophane, were used. Concentrations of 4 - 5000 µg/plate were tested with and without metabolic activation with rat S9 -mix. In no experiment the number of the induced revertants was the double of spontaneous mutations.