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EC number: 287-024-7 | CAS number: 85408-62-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-03-06 to 2008-06-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study is GLP compliant and follows OECD 201 guideline (Freshwater Alga and Cyanobacteria, Growth Inhibition Test). Full sample characterisation is provided. The analytical chemistry supporting this study was performed to GLP and confirms the identity of the test item but, the separate chemistry report has not been audited at the current time (04 November 2010).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): SLI (76) stripped
- Molecular formula (if other than submission substance): not applicable (mixture)
- Molecular weight (if other than submission substance): not applicable (mixture)
- Smiles notation (if other than submission substance): not applicable (mixture)
- InChl (if other than submission substance): not known
- Structural formula attached as image file (if other than submission substance): not applicable (mixture)
- Substance type:
- Physical state: cream coloured granular powder
- Analytical purity: 96%
- Impurities (identity and concentrations):
- Composition of test material, percentage of components:
- Isomers composition: not applicable (mixture)
- Purity test date:
- Lot/batch No.: S2849001
- Expiration date of the lot/batch:
- Stability under test conditions:
- Storage condition of test material:
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations (nominal): 0, 3, 10, 30, 100, 300 mg/L
- Concentrations (measured): 0.11, 0.37, 0.79, 7.86 and 135 mg/L
- Sampling method: Test concentrations at 0, 24, 48 and 72 h were determined by LC/MS.
- Sample storage conditions before analysis: Chemical analysis conducted immediately after sampling.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- A stock solution with a nominal concentration of 1000 mg/L was prepared by dissolving 1000 mg of test item in 1000 mL of purified water. The solution was heated to approximately 40 -50 oC whilst stirring continuously. Test media was prepared by dosing the appropriate volume of warm stock and ISO concentrate into purified water to give nominal concentrations of 0, 3, 10, 30, 100 and 300 mg/L.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Algae
- Strain: Pseudokirchneriella subcapitata
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa, SAMS Research Services Ltd, Dunstaffnage Marine Laboratory, Dunbeg, OBAN, Argyll, PA37 1QA, Scotland.
- Age of inoculum (at test initiation): No data - study conducted according to OECD 201 guideline
- Method of cultivation: No data - study conducted according to OECD 201 guideline
ACCLIMATION
- Acclimation period: No data - study conducted according to OECD 201 guideline
- Culturing media and conditions (same as test or not): ISO media (Reference: ISO 8692:1989 (E) Water Quality-Freshwater algal growth inhibition test with Selenastrum capricornutum and Scenedesmus subspicatus).
- Any deformed or abnormal cells observed: No data
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No data
Test conditions
- Hardness:
- No data
- Test temperature:
- 22-25 degrees C
- pH:
- In the control the pH at 0 and 72 h were 6.9 and 7.7, respectively.
In test item (nominal 3.0 mg/L) the pH at 0 and 72 h were 7.4 and 7.4, respectively.
In test item (nominal 10.0 mg/L) the pH at 0 and 72 h were 7.5 and 7.2, respectively.
In test item (nominal 30.0 mg/L) the pH at 0 and 72 h were 7.5 and 6.8, respectively.
In test item (nominal 100.0 mg/L) the pH at 0 and 72 h were 7.6 and 6.7, respectively.
In test item (nominal 300.0 mg/L) the pH at 0 and 72 h were 7.6 and 6.6, respectively. - Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal: 0, 3, 10, 30, 100, 300 mg/L
Total Measured: 0, 0.11, 0.37, 0.79, 7.86 and 135 mg/L
Dissolved measured: 0, 0.10, 0.31, 0.68, 2.00, 102 mg/L - Details on test conditions:
- Ask Jayne
For the definitive study a series of test solutions were prepared by diluting a 1000 mg/L stock solution with ISO media to give final test media concentrations of 0, 3, 10, 30, 100 and 300 mg/L. A calculated aliquot of Pseudokirchneriella subcapitata inoculum was transferred into each test solution to give a nominal algal density of 10E4 cells/mL. Cell concentration was determined at 48 and 72 hours by haemocytometer. Test concentrations were determined every 24 hours by Liquid Chromatography/Mass Spectroscopy (LC/MS).
Blank flasks were prepared by removing an aliquot of test media from each volumetric flask before the Pseudokirchneriella subcapitata inoculum was added. Glassware was not conditioned prior to the start of the test. Test flasks were placed in illuminated orbital incubators according to the randomised distribution numbers generated by the Random.bas computer software. Incubator temperatures were recorded at 24-hour intervals for the duration of the test. Light intensity was not recorded.
A 10 mL aliquot of each solution was provided to Chemical Analysis at 0, 24, 48 and 72 hours for determination of test item concentration. Test concentrations were determined by LC/MS by the Chemical Analysis.
The algal concentrations in the test media were determined at 0, 48 and 72 hours using a haemocytometer.
Incubator temperatures ranged between 22 and 25 oC throughout the experimental period. The pH of each test solution was measured at 0 hours. The pH of the remaining test media in the first replicate flask of each test concentration was measured at 72 hours. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 1.87 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 7.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- (total concentration)
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% fiducial interval (5.64, 11.56)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 2.88 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- (total concentration)
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 4.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% fiducial interval (3.66, 6.50)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.37 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- (total concentration)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.37 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- (total concentration)
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.31 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.31 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): Yes, 110-fold increase in the cell density of control cultures over 72 h
- Observation of abnormalities (for algal test):
- No data
- Other obsevations:
The test item has limited solubility in water and ISO media. There are significant losses of the test item over a 72 hr period. At 0 hours the measured concentration of each chain length dissolved in solution is close to the expected nominal concentration taking into account the chain length distribution of the test item. The concentrations of C8, C10 and C12 remain stable over the test period however there is a significant decrease in the concentration of chain lengths ≥ C14 in solution. Comparisons between solutions with and without algae reveal a significant decrease in the total concentratio remaining at 72 hours when algae is present (30% of the 100 mg/L solution remaining compared to 65% for the same concentration without algae). - Results with reference substance (positive control):
- No reference substance used
- Reported statistics and error estimates:
- 95% fiducial intervals given for 72-h EC50 (measured total and dissolved concentrations) - see results
Any other information on results incl. tables
Table 1: Cell densitites (cells/mL)
Flask code |
Mean measured conc (mg/L) |
Time (hours) |
||||
Total |
Dissolved |
0 |
48 |
72 |
||
A1 |
0.0 |
0.0 |
10000 |
272000 |
1111500 |
|
A2 |
0.0 |
0.0 |
10000 |
212000 |
760500 |
|
A3 |
0.0 |
0.0 |
10000 |
214000 |
934500 |
|
A4 |
0.0 |
0.0 |
10000 |
251000 |
1272000 |
|
A5 |
0.0 |
0.0 |
10000 |
279500 |
1165500 |
|
A6 |
0.0 |
0.0 |
10000 |
346500 |
1362000 |
|
B1 |
0.11 |
0.10 |
10000 |
283500 |
1122000 |
|
B2 |
0.11 |
0.10 |
10000 |
279500 |
1009500 |
|
B3 |
0.11 |
0.10 |
10000 |
318000 |
1296000 |
|
C1 |
0.37 |
0.31 |
10000 |
338500 |
1266000 |
|
C2 |
0.37 |
0.31 |
10000 |
209500 |
835500 |
|
C3 |
0.37 |
0.31 |
10000 |
326500 |
1161000 |
|
D1 |
0.79 |
0.68 |
10000 |
192500 |
482500 |
|
D2 |
0.79 |
0.68 |
10000 |
93500 |
393500 |
|
D3 |
0.79 |
0.68 |
10000 |
239000 |
473000 |
|
E1 |
7.86 |
2.00 |
10000 |
57000 |
157500 |
|
E2 |
7.86 |
2.00 |
10000 |
54000 |
177500 |
|
E3 |
7.86 |
2.00 |
10000 |
45500 |
153500 |
|
F1 |
135 |
102 |
10000 |
10000 |
11000 |
|
F2 |
135 |
102 |
10000 |
10000 |
14500 |
|
F3 |
135 |
102 |
10000 |
13000 |
11500 |
|
Table 2: 72 -h mean percentage inhibition of biomass and growth rates
Mean measured concentration (mg/L) |
Biomass |
Growth rate |
|
Total |
Dissolved |
|
|
0.11 |
0.10 |
-7.30 |
-1.48 |
0.37 |
0.31 |
-3.98 |
-0.27 |
0.79 |
0.68 |
49.44 |
18.14 |
7.86 |
2.00 |
84.89 |
41.78 |
135 |
102 |
99.71 |
95.92 |
Table 3: Summary of SLI(76) stripped concentrations during 72 -h study
Nominal concentration (mg/L) |
Measured concentration (mg/L) |
Mean measured concentration (mg/L)* |
||||||||
0 hours |
24 hrs |
48 hrs |
72 hrs |
|||||||
Total |
Dissolved |
Total |
Dissolved |
Total |
Dissolved |
Total |
Dissolved |
Total |
Dissolved |
|
3 |
2.35 |
2.02 |
<0.18 |
<0.19 |
<0.05 |
<0.05 |
<0.05 |
<0.05 |
0.11 |
0.10 |
10 |
10.1 |
7.91 |
3.04 |
1.95 |
<0.05 |
<0.05 |
<0.05 |
<0.05 |
0.37 |
0.31 |
30 |
36.2 |
29.2 |
17.3 |
11.6 |
<0.05 |
<0.05 |
<0.05 |
<0.05 |
0.79 |
0.68 |
100 |
114 |
89.8 |
71.1 |
42.1 |
18.8 |
<0.10 |
<0.05 |
<0.17 |
7.86 |
2.00 |
300 |
344 |
272 |
263 |
158 |
103 |
59.0 |
36.0 |
42.5 |
135 |
102 |
Table 4: Total concentrations of SLI(76) stripped in solution during definitive study according to chain length distribution.
Nominal concentration (mg/L) |
|
Measured concentration of chainlengths (mg/L) |
Total Measured Concentration (mg/L) |
|||||
Time (hrs) |
C8 |
C10 |
C12 |
C14 |
C16 |
C18 |
||
3 |
0 |
0.12 |
0.13 |
0.69 |
0.29 |
0.51 |
0.61 |
2.35 |
24 |
0.09 |
0.05 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.18 |
|
48 |
<0.01 |
0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
72 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
10 |
0 |
0.43 |
0.45 |
2.57 |
1.20 |
2.80 |
2.66 |
10.1 |
24 |
0.41 |
0.37 |
0.92 |
0.04 |
0.47 |
0.82 |
3.04 |
|
48 |
<0.01 |
0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
72 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
30 |
0 |
1.43 |
1.48 |
8.84 |
4.28 |
10.32 |
9.83 |
36.2 |
24 |
1.33 |
1.33 |
5.08 |
0.96 |
4.02 |
4.54 |
17.3 |
|
48 |
<0.01 |
0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
72 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
100 |
0 |
4.40 |
4.51 |
28.2 |
13.4 |
32.5 |
31.2 |
114 |
24 |
4.18 |
4.27 |
20.3 |
6.43 |
17.9 |
18.0 |
71.1 |
|
48 |
<0.01 |
0.02 |
0.04 |
0.95 |
5.98 |
11.8 |
18.8 |
|
72 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
300 |
0 |
13.1 |
13.5 |
84.7 |
41.9 |
97.2 |
93.1 |
344 |
24 |
13.0 |
13.4 |
71.8 |
30.5 |
70.9 |
63.7 |
263 |
|
48 |
12.0 |
9.27 |
9.77 |
3.61 |
16.0 |
52.0 |
103 |
|
72 |
6.24 |
1.22 |
<0.02 |
0.14 |
1.44 |
27.0 |
36.0 |
Table 5: Dissolved concentrations of SLI(76) stripped in solution during definitive study according to chain length distribution.
Nominal concentration (mg/L) |
|
Measured concentration of chainlengths (mg/L) |
Total Measured Concentration (mg/L) |
|||||
Time (hrs) |
C8 |
C10 |
C12 |
C14 |
C16 |
C18 |
||
3 |
0 |
0.12 |
0.13 |
0.68 |
0.29 |
0.31 |
0.49 |
2.02 |
24 |
0.10 |
0.05 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.19 |
|
48 |
<0.01 |
0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
72 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
10 |
0 |
0.44 |
0.45 |
2.62 |
1.04 |
1.72 |
1.64 |
7.91 |
24 |
0.43 |
0.41 |
0.97 |
0.01 |
<0.01 |
0.18 |
1.95 |
|
48 |
<0.01 |
0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
72 |
<0.01 |
0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
30 |
0 |
1.45 |
1.48 |
8.88 |
3.62 |
6.87 |
6.78 |
29.2 |
24 |
1.38 |
1.39 |
5.28 |
0.59 |
0.97 |
2.01 |
11.6 |
|
48 |
<0.01 |
0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
72 |
<0.01 |
0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
<0.05 |
|
100 |
0 |
4.46 |
4.56 |
28.2 |
11.1 |
21.1 |
20.4 |
89.8 |
24 |
4.12 |
4.28 |
20.1 |
3.30 |
4.15 |
6.10 |
42.1 |
|
48 |
<0.02 |
0.02 |
<0.02 |
<0.01 |
<0.02 |
<0.33 |
<0.10 |
|
72 |
<0.01 |
<0.01 |
<0.01 |
<0.01 |
0.16 |
1.02 |
<1.17 |
|
300 |
0 |
13.2 |
13.4 |
84.1 |
37.3 |
63.6 |
60.5 |
272 |
24 |
13.3 |
13.8 |
72.5 |
24.9 |
19.2 |
14.0 |
158 |
|
48 |
12.7 |
9.66 |
8.58 |
1.15 |
5.27 |
21.6 |
59.0 |
|
72 |
11.4 |
3.17 |
<0.01 |
0.14 |
1.50 |
26.3 |
42.5 |
Applicant's summary and conclusion
- Conclusions:
- In this study the effects of the test item SLI(76) stripped on the growth of the freshwater green alga Pseudokirchneriella subcapitata were tested. The EC50-values for specific growth rate inhibition (ErC50) and inhibition of yield (EyC50) after 72 h were >= 1.87 mg/L (dissolved measured concentration). The NOEC values were >= 0.31 mg/L (dissolved measured concentration).
- Executive summary:
This study was used to determine the 72-hour EC50 and NOEC (No Observed Effect Concentration) of SLI (76) stripped toPseudokirchneriella subcapitata.
SLI (76), provided by Unilever Mannheim was stripped of its fatty acid content via Soxhlet extraction at the Materials Science Laboratory, Trumbull, USA. The resulting material, SLI (76) stripped was used for this study.
Prior to testing the solubility of the test item and its individual actives were determined by Liquid Chromatography/Mass Spectroscopy (LC/MS) by the Chemical Analysis.
The test item has limited solubility in water and ISO media. There are significant losses of the test item over a 72 hr period. At 0 hours the measured concentration of each chain length dissolved in solution is close to the expected nominal concentration taking into account the chain length distribution of the test item. The concentrations of C8, C10and C12remain stable over the test period however there is a significant decrease in the concentration of chain lengths ≥ C14in solution. Comparisons between solutions with and without algae reveal a significant decrease in the total concentration remaining at 72 hours when algae is present (30% of the 100 mg/L solution remaining compared to 65% for the same concentration without algae).
For the definitive study a series of test solutions were preparedby diluting a 1000 mg/L stock solutionwith ISO media to give final test media concentrations of 0, 3, 10, 30, 100 and 300 mg/L. A calculated aliquot ofPseudokirchneriella subcapitatainoculum was transferred into each test solution to give a nominal algal density of 104cells/mL. Cell concentration was determined at 48 and 72 hours by haemocytometer. Test concentrations were determined every 24 hours by Liquid Chromatography/Mass Spectroscopy (LC/MS).
In this study the effects of the test item SLI(76) stripped on the growth of the freshwater green alga Pseudokirchneriella subcapitata were tested. The EC50-values for specific growth rate inhibition (ErC50) and inhibition of yield (EyC50) after 72 h were >= 1.87 mg/L (dissolved measured concentrations). The NOEC values were >= 0.31 mg/L (dissolved measured concentrations).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.