4-chloro-3,5-dinitro-α,α,α-trifluorotoluene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-chloro-3,5-dinitro-α,α,α-trifluorotoluene

EC Number:

206-889-3

EC Name:

4-chloro-3,5-dinitro-α,α,α-trifluorotoluene

Cas Number:

393-75-9

Molecular formula:

C7H2ClF3N2O4

IUPAC Name:

4-chloro-3,5-dinitro-α,α,α-trifluorotoluene

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/l

Test temperature:

20-21°C

pH:

8.0-8.1

Nominal and measured concentrations:

Due to the low water solubility a supersaturated stock suspension of the test item with a nominal concentration of 100 mg/l was continuously stirred at room temperature in the dark over 96 hours and filtered. The dilutions 1:100, 1:222, 1:454, 1:1000 and 1:2222 of the filtrate were used as test media.
The mean analytically determined test item concentration in the analyzed test media samples (dilutions 1:454, 1:222 and 1:100) amounted to 0.18, 0.38 and 0.88 mg/l at the start of the test. The values found at the end of the test ranged from 98-106% of the initially measured values.
The lowest test concentrations (dilutions 1:1000 and 1:2222) were not analysed since below the 48-hr NOEC.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.6 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 0.5-0.7 mg/l

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.38 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

1.9 mg/L

Nominal / measured:

estimated

Details on results:

All biological test results are related to the total mean measured test item concentrations of 0.19 mg/l (1:454), 0.38 mg/l (1:222) and 0.87 mg/l (1:100), calculated as the average over all measurements per test concentration during the test period.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes