9-ethylcarbazole

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: pre-guideline study and similar to OECD TG 203, but limited documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

9-ethylcarbazole

EC Number:

201-660-4

EC Name:

9-ethylcarbazole

Cas Number:

86-28-2

Molecular formula:

C14H13N

IUPAC Name:

9-ethyl-9H-carbazole

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Idus idus

Details on test organisms:

- Common name: Golden Orfe (idus idus melanotus)
- Length at study initiation: 4.2 cm
- Weight at study initiation: 2.7 g

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions: 22 ± 1°C in test water

Study design

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

96 h

Test conditions

Nominal and measured concentrations:

1 mg/L, 10 mg/L, 100 mg/L and 500 mg/L (nominal)

Details on test conditions:

- Aeration: ≥ 7 mg/L O2
- No. of organisms per vessel: 10

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 - < 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Concentration [mg/L]	Mortalities
	24 h	48 h	72 h	96 h
500	10	10	10	10
100	10	10	10	10
10	9	10	10	10
1	0	0	0	0

Applicant's summary and conclusion

Conclusions:

LC50(96 h): > 1 mg/L - < 10 mg/L, log Pow (see 4.7.): 4.47

Directive 67/548/EEC: Acute aquatic toxicity N, R 51/53
Regulation (EC) No 1272/2008: Chronic aquatic hazard Category 2

Executive summary:

The 1 mg/L solution of the test item didn´t show any signs of toxicity nor lethality at all observation time points up to 96 hours. Therefore, the NOEC and LC₀were laid down as >= 1 mg/lL. The 10 mg/L solution resulted in 100 % mortality of the fish. Therefore the LC₅₀(96) is < 10 mg/L.