Cyclohexanone, 4-methoxy-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16 Jun - 10 Jul 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

GC

Details on sampling:

To aliquots of the samples from the biological test, 2 pellets potassium hydroxide and about 4.5 g sodium sulphate were added. These mixtures were diluted with water to measuring concentration if necessary and were extracted with diethylether after addition of internal standard and analysed by GC and MS-detection.

Vehicle:

no

Details on test solutions:

A direct weighing was prepared to give the desired test concentration. To achieve this 501.7 mg of the test item were added to 5 litres of dilution water and treated with an ultra turrax for 60 seconds at 8000 rpm.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Source: Aqua KloGer (Germany)
Date supplied: 2006-03-03
Acclimitisation: Stock held since 2006-03-03 and acclimatised to the test conditions since then. Temperature: 20 - 24 °C, dissolved oxygen: > 5 mg/1, Feeding: Commercial fish food, daily. Discontinued 24 h prior to test. Mortalities during acclimatisation period: < 5 % Medication: none, Mean length: 3.4 cm (S.D. = 0.26 cm).

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

13.8 °dH (= 246.33 mg/L Ca2CO3)

Test temperature:

mean +/- 1 °C (in the temperature range 20 - 24 °C)

pH:

7.6 to 7.9

Dissolved oxygen:

7.8 to 8.4 mg/L

Nominal and measured concentrations:

100 mg/L nominal

Details on test conditions:

Test vessels: glass aquaria holding 5 L of test media
Experimental design: 1 test concentration plus 1 control; 10 animals per test concentration; no feeding during the exposure period; static system
Method of initiation: animals were placed in prepared media
Loading: 0.64 g body weight (wet weight) per litre
Photoperiod: 16 h light: 8 h dark
Aeration: gentle aeration via narrow glass tubes
Method of administration: direct weighing
Medium renewal: none
Duration of exposure: 96 h
Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, subjective assessments were made on the type and incidence of sub-lethal effects compared with control fish.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

46 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No toxic effects against fish at a concentration of 100 mg/L. All results are expressed in terms of nominal concentrations. Recovery rates correspond to 104.9 % of nominal value at 0 hours, to 100.7 % of nominal value at 24 hours and to 89.8 % of nominal value at 96 hours.

Sublethal observations / clinical signs:

Some test fish had a somewhat larger size than the one recommended in the test guideline for the zebra fish. This deviation is not regarded to be relevant to the results.

 

Validity criteria for the measurement of the fish toxicity

Target condition according to guideline:	Actual condition according to the study:	Validity criteria met:
In the control(s) (dilution water control, solvent control), the mortality should not exceed 10% (or one fish, if fewer than 10 control fish are tested) at the end of the exposure.	No fish died during exposure.	Yes
All fish should be held in the laboratory for at least 9 days before they are used for testing. The first 48 hours constitute a settling-in period. Then, fish should be acclimatised for at least 7 days (48 hours settling-in + 7 days acclimatisation = 9 days) in water similar to test water immediately before the start of the test.	Stock held since 2006-03-03 and acclimatised to the test conditions since (test performed 2006-06-16).	Yes
During the acclimatisation period, mortalities are recorded, and the following criteria are applied: - mortalities of greater than 10% of the population in seven days: reject the entire batch; - mortalities between 5 and 10% of the population: acclimatisation is continued for seven additional days; and if there is more than 5% mortality during the second seven day period, reject the entire batch; - mortalities of less than 5% of population in seven days: accept the batch.	Mortalities during acclimatisation period: < 5 %	Yes
Temperature: the water temperature should not differ by more than 2°C	mean +/- 1 °C (in the temperature range 20 - 24 °C)	Yes
Duration: 96 hours.	Duration: 96 hours.	Yes

 

Validity criteria fulfilled:

yes

Remarks:

See 'Any other information on results incl. tables'.

Conclusions:

No toxic effects against fish at a concentration of 100 mg/L.

Executive summary:

No toxic effects against fish at a concentration of 100 mg/L.

Description of key information

No toxic effects against fish at a concentration of 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L