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Diss Factsheets
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EC number: 455-560-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- see Principles of method if other than guideline, The study integrity was not adversely affected by the deviations.
- Principles of method if other than guideline:
- There were deviations from the test protocol.
1. During the first limit test samples were taken from two different points in the test solution rather than from the middle of the test solutions. Evaluation: this was triggered by the observation variation in measured conc. during the range finding test and was meant to provide relevant information concerning homogeneity of the test solution.
2. During the final limit test, reserve samples were taken even duplicate samples were taken for analysis. Evaluation: this was triggered by the analytical results of the former studies and was aimed at providing relevant information if needed.
3. During the final limit test, recording of the effects was done after 6 ¼ hours instead of 4 hours. Evaluation: Since no effects were observed, this did not have an impact on the test results.
The study integrity was not adversely affected by the deviations. - GLP compliance:
- yes
- Specific details on test material used for the study:
- Clear colourless liquid, purity 96.5%.
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- The stock solution was prepared at 100 mg/l. The test substance was dissolved by magnetic stirring. Lower concentrations were prepared by dilution of the stock solution.
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Carp (Cyprinus carpio, Cyprinidae), Zodiac, proefacc, “De Haar Vissen”, Wageningen University and Research Centre, the Netherlands
Mean length:
Range finding test: 3.0 ± 0.2 cm
First limit test: 2.9 ± 0.2 cm
Second limit test: 2.5 ± 0.1 cm
Mean weight:
Range finding test: 0.77 ± 0.14 g
First limit test: 0.73 ± 0.15 g
Second limit test: 0.39 ± 0.13 g
Ten fish of the batch used for the test were weighed and measured prior to the start of the test - Test type:
- static
- Water media type:
- other: In ISO medium, formulated using Milli-Ro water
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 250 mg CaCO3 per litre
- Test temperature:
- 20.4 - 21.2 oC
- pH:
- 7.6-8.1
- Dissolved oxygen:
- 6.7-9.3
- Nominal and measured concentrations:
- Nominal concentrations:
Range finding test: Concentrations of 0.1- 100 mg/l increasing with a factor 10.
First limit test: Blank control and 100 mg/l
Final limit test: Blank control, 100 mg/l and test solution without fish.
Measured concentrations:
Range finding test: the measured conc in the 100 mg/l conc. at t=0 94 mg/l, t=24 60mg/l and t=96 83 mg/l. Reserve samples at t=0 75 mg/l, t=24 79 mg/l and t=96 65 mg/l. After measuring the reserve samples it was assumed that the variability of the analytical results were related to different days of analysis rather than to instability of the test substance.
The first limit test: t=0 71-74 mg/l t=96 decreased to 31 mg/l. Therefore this test did not meet acceptability criteria and a second limit test was performed.
Final limit test:
The test substance was stable
Arithmetic mean was used. - Details on test conditions:
- Range finding test: 10 liter vessels, all glass containing 7 liters of test solution.
First limit test: 20 liter vessels, all glass containing 15 liters of test solution.
Final limit test: 10 liter vessels, all glass containing 9 liters of test solution.
Range finding test:
3 fish per conc.
First and final test:
7 fish per conc. and control - Reference substance (positive control):
- yes
- Remarks:
- pentachlorophenol
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: no clinical effects, no mortality
- Remarks on result:
- other: also 24, 48 and 72 h
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: No clinical effects observed, no mortality
- Remarks on result:
- other: also 24, 48 and 72 h
- Details on results:
- No clinical effects were observed, no mortality
- Results with reference substance (positive control):
- Zero mortality at 0.1 mg/l (nominal); zero through 96 h
100 % mortality at 0.22 mg/l (nominal); zero at 2.25 h, 100 % from 24 h
100 % mortality at 0.46 mg/l (nominal); zero at 2.25 h, 100 % from 24 h - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present test Pentamethyl-trioxepane induced no visible effects in carp at 100 mg/l. The 96h-LC50. was > 100 mg/l based on analytically confirmed nominal concentrations. Based on LC50>100 mg/l, pentamethyl-trioxepane is not classified.
- Executive summary:
The 96h-LC50. was > 100 mg/l based on analytically confirmed nominal concentrations.
Reference
Description of key information
Under the conditions of the present test Pentamethyl-trioxepane induced no visible effects in carp at 100 mg/l. The 96h-LC50. was > 100 mg/l based on analytically confirmed nominal concentrations. Based on LC50>100 mg/l, pentamethyl-trioxepane is not classified.
Key value for chemical safety assessment
Additional information
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