Phosphoric acid, mono- and di-C16-18 (even numbered) alkyl esters, salts with 2,2'-iminodiethanol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Remarks:

See Justification of Read Across

Adequacy of study:

supporting study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

See the Read Across justification document

Reason / purpose for cross-reference:

read-across: supporting information

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

DIN 38412-27 (Pseudomonas putida Zellvermehrungshemmtest)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Name: Phosphoric acid, mixed decyl and octyl esters, compds. with diethanolamine (Phosphorsaeure (C8-C10-Alkyl)-ester, DEA-Salz)
CAS No.: 68425-57-0 (68186-45-8)
Physical state: white solid at 20 °C (turbid, non-pourable paste)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Culture media: H5-Water, Glucose

Test organisms (species):

Pseudomonas putida

Details on inoculum:

Pseudomonas putida MIGULA, Stamm Berlin 33/2 (DSM 50026)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Details on test conditions:

Age of susension at beginning of the test: 2 d
Test volume: 100 mL
Conentrations: 300, 100, 3000 mg test item / L

Reference substance (positive control):

not specified

Key result

Duration:

3 h

Dose descriptor:

EC0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (total fraction)

Basis for effect:

inhibition of total respiration

Key result

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

1 030 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Validity criteria fulfilled:

yes

Conclusions:

The test item had no inhibitory effect on the total oxygen uptake of activated sludge microorganisms after 3 hours at 1000 mg/l. The EC0 is therefore determined to be 1000 mg/L. The EC10 for total oxygen uptake was calculated at a test item concentration of 1030 mg/L.

Executive summary:

The objectives of this study were to determine the effects of test item on microorganisms respiration under laboratory conditions. The oxygen uptake rate was measured after 3 hours of exposure. The total oxygen uptake rate of the same microorganisms in the presence of various concentrations of the test item under otherwise identical conditions was also measured. The GLP study was performed according to DIN 38412, Part 27. The test item had no inhibitory effect on the total oxygen uptake of microorganisms after 3 hours at a concentration of 1000 mg/l. The EC0 is therefore determined to be 1000 mg/L. The EC10 for total oxygen uptake was calculated at a  test item concentration of 1030 mg/L.

Description of key information

The toxicity to microorganisms was evaluated with results of a similar substance, tested according to DIN 38412-27 (Pseudomonas putida Zellvermehrungshemmtest). See the Justification of the Read Across.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

1 030 mg/L