Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-588-9 | CAS number: 7646-78-8
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Genotoxicity studies on selected organosilicon compounds: in vitro assays
- Author:
- Isquith, A., Matheson, D., and Slesinski, R.
- Year:
- 1 988
- Bibliographic source:
- Fd. Chem. Toxic. 26, 255-261
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The procedure by Clive and Spector (Mutat. Res., 31, 17-29, 1975) was modified.
Fischer’s medium for Leukemic cells of mice with 10% horse serum and sodium pyruvate and those with 20% horse serum, sodium pyruvate and 0.37% agar were used for maintaining and cloning, respectively. - GLP compliance:
- no
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- Hydrogen chloride
- EC Number:
- 231-595-7
- EC Name:
- Hydrogen chloride
- Cas Number:
- 7647-01-0
- Molecular formula:
- ClH
- IUPAC Name:
- chloride
- Test material form:
- gas
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Mouse lymphoma cell line L5178Y TK+/-
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Vehicle / solvent:
- water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: Without activation : Ethylmetanesulfonate 0.5 uL/mL Witht activation : Dimethylnitrosoamine 0.3 uL/mL
Results and discussion
Test results
- Key result
- Species / strain:
- other: Mouse lymphoma cell line L5178Y TK+/-
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Cytotoxic concentration: z With metabolic activation: 0.8 uL/mL (100% growth inhibition) z Without metabolic activation: ca 1.0 uL/mL (12.5% growth inhibition at 0.8 uL/mL)
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: -
Applicant's summary and conclusion
- Conclusions:
- enotoxic effects (positive, negative, unconfirmed, dose-response, equivocal)z With metabolic activation: Negative z Without metabolic activation: Negative
Negative result was obtained in the systems.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
