Esterification products of Guerbet alcohols, C24-26, branched and cyclic with benzene-1,2,4-tricarboxylic acid 1,2-anhydride (3:1)

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Further information are provided in the read-across justification document.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 3.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: No toxicity up to the highest concentration tested.

Conclusions:

The test substance was not toxic under test conditions in the range of water solubility.

Description of key information

In the available key study for the analogue substance no toxicity under test conditions in the range of water solubility was reported.

Additional information

In the key study the inhibitory effect of the analogue substance 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters on the growth of the green alga Scenedesmus subspicatus, strain CHODAT (86.81 SAG) had been tested in accordance with OECD Guideline for Testing of Chemicals No.201 and EEC Methods for Determination of Ecotoxicity, Annex to Directive 92/69/EEC.

The test algae were exposed to 6 concentrations of 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters, ranging from 0.24 to 3.5 mg/L. As the test substance was poorly soluble in water, a 1 g/L mixture with deionised water was prepared. This mixture was stirred for 18 hours and filtrated afterwards. The filtrate was analysed and showed an initial content of 3.9 mg/L DOC and was used as stock solution. The cultures were incubated on sterile ventilated Erlenmeyer flasks on light tables at 24±2 °C. Growth was monitored by determining the cell number photometrically at a wave length of 685 nm after 24, 48 and 72 hours. No inhibitory effects on the growth of the green alga were observed.

On the basis of cell growth, a mean effective concentration was calculated to be EbC50 (72 h) > 3.5 mg/L.

On the basis of the growth rate, a mean effective concentration was calculated to be ErC50 (0 -72 h) > 3.5 mg/L.

The NOEC value was >= 3.5 mg/L (on basis of cell growth).

In a supporting study the effect of the test item on the growth of Pseudokirchneriella subcapitata has been investigated and gave EL50 values of greater than 100 mg/L loading rate WAF. The No Observed Effect Loading Rate was 100 mg/L loading rate WAF.

Therefore in both tests the analogue substance was not toxic under test conditions in the range of water solubility.