3-(2,2-dimethyl-3-hydroxypropyl)toluene

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19th until 23rd of October 1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no analytical verification, but no loss of the test substance was seen in the algae test and as well in the suporting fish study. Thus, the results based on nominal values are reliable

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

GLP compliance:

yes

Analytical monitoring:

no

Remarks:

No loss of the test substance was seen in the algae test and as well in the suporting fish study.

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: STRAUSS
- Age at study initiation (mean and range, SD): not more than 24 hours at beginning of test
- Source: Bundesgesundheitsamt, Berlin, Germany
- Food type: The source culture is fed with fresh algae (Chlorella vulgaris)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Total hardness of dilution water: 19.1°d
carbonate hardness of dilution water: 2.8°d

Test temperature:

20.3 - 20.4°C

pH:

7.1 (50 mg/L) - 8.05 (1 mg/L)

Dissolved oxygen:

89 - 95% oxygen concentrations

Nominal and measured concentrations:

Nominal concentrations: 1, 5, 10, 15, 25, 37.5, 50 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 250 mL glass beaker, fill volume: 40 mL
- No. of organisms per vessel: 20 animals
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


OTHER TEST CONDITIONS
- Photoperiod: 8 hours darkness, 16 hours light

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

19 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

37.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: No mortality in the control
- Immobilisation of control: No immogilisation of in the control

Reported statistics and error estimates:

The percentage of immobilised animals at each test concentration is first calculated, and then converted to probits using the values below. These data are plotted against the log of the concentration of test substance; the linear fit is carried out by regression analysis, whereby individual points which obviously deviate from the general trend are excluded.

Results of the acute immobilisation test

Cumulative immobilisation at 24h and 48h, cummulative percentage of immobilisation in % ( )

Test concentrations [mg/L]	Immobilised Daphnia [24h]	Immobilised Daphnia [48h]
50	20 (100)	20 (100)
37.5	13 (65)	20 (100)
25	6 (30)	12 (60)
15	5 (25)	8 (40)
10	2 (10)	5 (25)
5	0 (0)	1 (5)
1	0 (0)	0 (0)
0 (CONTROL)	0 (0)	0 (0)

Validity criteria fulfilled:

yes

Conclusions:

In the acute immobilisation study with Daphnia magna according to OECD 202 an EC50(48h) of 19 mg/L was determined.

Description of key information

EC50(48h): 19 mg/L based on immobilisation of Daphnia magna (OECD 202, static).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

19 mg/L

Additional information

One experimental study is available investigating the short-term effects of the substance to aquatic invertebrates. The study was performed according to OECD 202 (1984) (GLP) with the water flea Daphnia magna. Nominal concentrations of 1, 5, 10, 15, 25, 37,5 and 50 mg/L were tested under static conditions. Test concentrations were not analytically verified, but the substance is expected to be stable in the test medium which was proved in the algae and fish tests. Immobilisation of D. magna was observed after 48 h resulting in an EC50 (48 h) of 19 mg/L based on nominal concentrations.