Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 950-170-3 | CAS number: -
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The study was conducted between 02 January 2019 and 04 January 2019.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- (2008)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- (2001)
- Deviations:
- no
- GLP compliance:
- yes
- Type of method:
- HPLC estimation method
- Specific details on test material used for the study:
- Identification: PG-RAW-0004
Appearance/physical state: clear, colorless liquid
Storage conditions: approximately 4 °C in the dark - Radiolabelling:
- no
- Test temperature:
- 30°C
- Details on study design: HPLC method:
- Performance of the Test
Preparation of sample solution
Test item (0.5505 g) was diluted to 50 mL with methanol, to give a concentration of 1.10 x 10^4 mg/L.
Preparation of dead time solution (ultraviolet detector solution)
The ultraviolet detector dead time was determined by measuring the retention time of thiourea (purity* >99%, 28 mg/L solution in mobile phase).
Preparation of dead time solution (refractive index detector solution)
The refractive index detector dead time was determined by measuring the retention time of thiourea (purity* >99%, 1.08 x 10^4 mg/L solution in mobile phase).
Preparation of reference standard solutions
Solutions of reference standards were prepared in methanol.
Determination of retention time
The sample, dead time and reference standard solutions were injected in duplicate using the following HPLC parameters:
HPLC System : Agilent Technologies 1200 Series, incorporating autosampler and workstation
Detector types : ultraviolet (UV) at 210 nm
refractive index (RI), positive polatrity at 35 °C
Column : XSelect HSS cyano 5 µm (150 x 4.6 mm id)
Column temperature : 30 ºC
Mobile phase : methanol: water (55:45 v/v)
pH of mobile phase : 7.0
Flow-rate : 1.0 mL/min
Injection volume : 10 µL - Key result
- Type:
- log Koc
- Value:
- >= 2.16 - <= 3.18 dimensionless
- pH:
- 7
- Temp.:
- 30 °C
- Details on results (HPLC method):
- Overall result:
Log10 Koc range: 2.16 to 3.18
Adsorption coefficient range: 144 to 1.50 x 10^3 - Validity criteria fulfilled:
- yes
- Conclusions:
- The adsorption coefficient (Koc) range of the test item has been determined to be 144 to 1.50 x 10^3, log10 Koc 2.16 to 3.18.
- Executive summary:
The adsorption coefficient range of PG-RAW-0004 has been determined to be 144 to 1.50 x 103, log10 Koc 2.16 to 3.18. The determination was performed using an HPLC method designed to be compatible with Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001.
Reference
Calibration
The retention times of the dead time and the retention times, capacity factors (k') and log10 Kocvalues for the reference standards are shown in the following tables:
Ultraviolet (UV) Detector Dead Time
Dead Time |
Retention Time (mins) |
Mean Retention Time (mins) |
|
Injection 1 |
Injection 2 |
||
Thiourea |
1.987 |
1.987 |
1.987 |
Refractive Index (RI) Detector Dead Time
Dead Time |
Retention Time (mins) |
Mean Retention Time (mins) |
|
Injection 1 |
Injection 2 |
||
Thiourea |
2.182 |
2.189 |
2.186 |
Calibration Standards Ultraviolet (UV) Detector Data
Standard |
Retention Time (mins) |
Capacity Factor (k') |
Log10k' |
Log10Koc |
||
Injection 1 |
Injection 2 |
Mean |
||||
Acetanilide |
2.619 |
2.619 |
2.619 |
0.318 |
-0.498 |
1.25 |
Phenol |
2.498 |
2.498 |
2.498 |
0.257 |
-0.591 |
1.32 |
Atrazine |
3.523 |
3.528 |
3.525 |
0.774 |
-0.111 |
1.81 |
Isoproturon |
3.727 |
3.722 |
3.724 |
0.874 |
-5.84 x 10-2 |
1.86 |
Triadimenol |
4.806 |
4.806 |
4.806 |
1.418 |
0.152 |
2.40 |
Linuron |
4.913 |
4.913 |
4.913 |
1.472 |
0.168 |
2.59 |
Calibration Standards Ultraviolet (UV) Detector Data – Continued
Standard |
Retention Time (mins) |
Capacity Factor (k') |
Log10k' |
Log10Koc |
||
Injection 1 |
Injection 2 |
Mean |
||||
Naphthalene |
4.320 |
4.315 |
4.317 |
1.172 |
6.91 x 10-2 |
2.75 |
Endosulfan-diol |
5.845 |
5.845 |
5.845 |
1.941 |
0.288 |
3.02 |
Fenthion |
6.997 |
6.997 |
6.997 |
2.521 |
0.402 |
3.31 |
a-Endosulfan |
9.519 |
9.514 |
9.517 |
3.789 |
0.578 |
4.09 |
Phenanthrene |
7.604 |
7.604 |
7.604 |
2.826 |
0.451 |
4.09 |
Diclofop-methyl |
10.588 |
10.374 |
10.481 |
4.274 |
0.631 |
4.20 |
DDT |
19.101 |
19.145 |
19.123 |
8.622 |
0.936 |
5.63 |
Adsorption Coefficient
The retention times, capacity factor and log10Kocvalues determined for the test item sample solution are shown in the following table:
Test Item Data
Peak Number |
Injection |
Retention Time[1](mins) |
Capacity Factor (k') |
Log10k' |
Log10Koc |
Mean |
Adsorption Coefficient |
1 |
1 |
3.881 |
0.853 |
-6.89 x 10-2 |
2.16 |
2.16 |
144 |
2 |
3.874 |
0.850 |
-7.07 x 10-2 |
2.16 |
|||
2 |
1 |
4.982 |
1.41 |
0.148 |
2.83 |
2.83 |
677 |
2 |
4.975 |
1.40 |
0.147 |
2.83 |
|||
3 |
1 |
5.803 |
1.82 |
0.260 |
3.18 |
3.18 |
1.50 x 103 |
2 |
5.796 |
1.82 |
0.259 |
3.17 |
Overall result:
Log10Kocrange: 2.16 to 3.18
Adsorption coefficient range: 144 to 1.50 x 103
Percentage area normalization data is shown in the following table:
Test Item Percentage Area Normalization Data
Peak Number |
Percentage Area Normalization |
||
Injection 1 |
Injection 2 |
Mean |
|
1 |
19.38 |
19.48 |
19.43 |
2 |
79.79 |
79.69 |
79.74 |
3 |
0.82 |
0.83 |
0.83 |
[1] The refractive index (RI) detector test item retention times were corrected for the internal volume of the HPLC system between the RI detector and the ultraviolet (UV) detector (0.199 mL, equivalent to 0.199 minutes) prior to calculation of the capacity factor values.
Description of key information
The adsorption coefficient range of PG-RAW-0004 has been determined to be 144 to 1.50 x 103, log10 Koc 2.16 to 3.18. The determination was performed using an HPLC method designed to be compatible with Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001.
Key value for chemical safety assessment
- Koc at 20 °C:
- 1 500
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
