Registration Dossier
Registration Dossier
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EC number: 251-073-2 | CAS number: 32509-66-3
Endpoint summary
Administrative data
Description of key information
Additional information
The toxicity of Hostanox O 3 on reproduction of earthworms was determined according to the principles of OECD 222 (2004). The study was conducted under static conditions over 8 weeks with five different application rates of 62.5 - 125 - 250 - 500 and 1000 mg product/kg soil dry weight, which were mixed into artificial soil containing 5% peat. Sexually mature earthworms were inserted into 8 control as well as solvent control replicates and into 4 replicates per test item concentrations (10 earthworms per replicate). The earthworms had an individual body weight ranging between 0.36 and 0.58 g at test initiation.
Under the conditions of this study,Hostanox O 3 did not induce evident mortality (10%) and no pathological symptoms of adult earthworms after an exposure of 28 days in all treatment rates. As there were no statistical differences determined between the control and the solvent control for the body weight and the reproduction rate, the controls were pooled. Compared to the pooled control, there were no statistically significant differences in earthworm body weights observed. After further four weeks the reproduction rate (average number of juveniles) was 148 in the pooled control and ranged between 127 and 181 in the treatment rates. Compared to the pooled control, there was no statistically significant difference in the level of earthworm reproduction across all treatments.
Overall, the NOEC of Hostanox O 3 concerning mortality, biomass and reproduction of earthworms was determined to be at 1000 mg product/kg soil dry weight.
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