Tetrasodium tripotassium (.my.-((2,2'-((3-imino-6-sulfonatophenyl-(N-methyl)-imino)bis((6-chloro-1,3,5-triazin-4,2-diyl)imino(2-hydroxy-5-sulfonato-3,1-phenylen)azo(phenylmethylen)azo))bis(4-sulfonatobenzoato))-dicuprate(7-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03 - 05 Nov 1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

No analytical dose verification, no substance purity reported

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

A stock solution of 500 mg/L was prepared by direct dilution of the test substance in test medium. Serial dilutions were prepared from the stock solution. To each test vessel, 10 ml of the respective dilution were added.

Test type:

static

Water media type:

freshwater

Remarks:

Artificial freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.70 +/- 0.50 mmol/L

Test temperature:

292-294K

Dissolved oxygen:

>2 mg/L

Nominal and measured concentrations:

- Nominal: 0, 31.25, 62.50, 125, 250 and 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: reagent tubes with flat bottoms
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 5 Daphnia / test vessel

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 450 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the nominal 48-h EC50 was 450 mg/L.

Executive summary:

A study was conducted to determine the toxicity of the test substance to water flea (Daphnia magna) according to EU Method C.2, in compliance with GLP. Groups of 20 Daphnia (4 per replicate vessel) were exposed for 48 h under static conditions to nominal concentrations of 0, 31.25, 62.50, 125, 250 and 500 mg/L. Inability to swim (after gentle agitation of the test vessels, if necessary) was determined at the beginning and after 3, 6, 24 and 48 h. No analytical dose verification was conducted. Under the study conditions, the nominal 48 h EC50 was 450 mg/L and the 48 h EC0 was 250 mg/L (Jatzek, 1989).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

450 mg/L

Additional information

A study was conducted to determine the toxicity of the test substance to water flea (Daphnia magna) according to EU Method C.2, in compliance with GLP. Groups of 20 Daphnia (4 per replicate vessel) were exposed for 48 h under static conditions to nominal concentrations of 0, 31.25, 62.50, 125, 250 and 500 mg/L. Inability to swim (after gentle agitation of the test vessels, if necessary) was determined at the beginning and after 3, 6, 24 and 48 h. No analytical dose verification was conducted. Under the study conditions, the nominal 48 h EC50 was 450 mg/L and the 48 h EC0 was 250 mg/L (Jatzek, 1989).