Bromacil

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 1986

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Bromacil

EC Number:

206-245-1

EC Name:

Bromacil

Cas Number:

314-40-9

Molecular formula:

C9H13BrN2O2

IUPAC Name:

5-bromo-3-sec-butyl-6-methylpyrimidine-2,4(1H,3H)-dione

Specific details on test material used for the study:

Purity: 96.6%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Dissolved oxygen and pH were measured in the H2O control and in the low, medium, and high exposure concentrations at the beginning and end of the exposure.

Test solutions

Vehicle:

yes

Details on test solutions:

The test material was prepared as a 0.333 mg/mL stock solution in laboratory Milli-Q® water. It was then diluted with hard reconstituted water to yield the desired nominal exposure concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Ten daphnids less than 24 hours old

Study design

Test type:

static

Total exposure duration:

49.5 h

Test conditions

Hardness:

181 mg/L as CaCO3

Test temperature:

20.1 - 20.2 °C

pH:

8

Dissolved oxygen:

8.4 - 8.6

Nominal and measured concentrations:

11.2, 14.5, 20, 27, 35.5, 47, 62, 83, 111 and 148 mg/L

Details on test conditions:

Immobility counts and observations were made at 24 and 48 hours after the exposure was initiated.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

119 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Bromacil has been tested for static acute toxicity to Daphnia magna during a 48-hour exposure period. Under the conditions of this test, bromacil was found to exhibit slight acute toxicity.

Applicant's summary and conclusion

Conclusions:

Bromacil exhibited slight acute toxicity to Daphnia magna under static unaerated test conditions during a 48-hour exposure period.
The 48-hour EC50 was 119 mg/L. The 95% confidence interval on the EC50 could not be calculated.