Propane-1,3-diyl bis(4-aminobenzoate)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Details on sampling:

Chemical analysis of the test item concentrations in the test solutions was performed to determine
exposure concentration and stability of the test item during exposure. Substance-specific analysis
was performed at the test site menal GmbH.
After 0 h, 24 h and 48 h exposure, 2 samples were taken from the test vessels of A, B, C, D, E and
NC without daphnids.
With every sampling 5 mL of the test solution were taken and filled into the 12 mL glass vials. For
stabilisation 5 ml acetonitril was submitted. This resulted in a dilution of test solution by factor 2.
The samples were kept in the freezer at ≤ -18°C. After the experimental phase one sample of each
sampling was sent to the analytical laboratory. The second sample was kept in the freezer as
retain sample until finalization of the study. Proper departure and arrival of the samples were
documented.

Test organisms (species):

Daphnia magna

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Details on test conditions:

The test organism Daphnia magna Straus originates from the clone breeding of the Federal
Environment Agency, department IV 2.4, Berlin, and has been cultivated at Hydrotox GmbH since
October 2012. The daphnids are cultured at 20 ± 2 °C with 16 h light : 8 h dark and placed into
fresh Elendt M4 medium twice a week. They are kept at approx. 10 animals / 200 mL and fed on
workdays with Desmodesmus subspicatus algae corresponding to 0.1 mg C × daphnid-1 × day-1.
The test organisms used were 1.5 – 22.75 h old at the start of the test.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

5.724 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

2.431 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2.29 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

EC50 (48 h): 5.724 mg/L, 95 % Confidence Limit (CL): 4.530 – 8.100 mg/L
EC10 (48 h): 2.431 mg/L, 95 % Confidence Limit (CL): 1.441 – 3.184 mg/L
NOEC: 2.292 mg/L (48 h)

Executive summary:

EC50 (48 h): 5.724 mg/L, 95 % Confidence Limit (CL): 4.530 – 8.100 mg/L

EC10 (48 h): 2.431 mg/L, 95 % Confidence Limit (CL): 1.441 – 3.184 mg/L

NOEC: 2.292 mg/L (48 h)

Description of key information

EC50 (48 h): 5.724 mg/L, 95 % Confidence Limit (CL): 4.530 – 8.100 mg/L

EC10 (48 h): 2.431 mg/L, 95 % Confidence Limit (CL): 1.441 – 3.184 mg/L

NOEC: 2.292 mg/L (48 h)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

5.724 mg/L