Phosphoric acid, mono- and di-isotridecyl esters, compd. with 2,2'-iminobis[ethanol]

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Read across study

Justification for type of information:

Refer to the section 13 of the IUCLID dataset for details on the read across justification. The toxicity to microorganisms study with the read across substance is considered sufficient to fulfil the information requirements.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EC Directive 88/302/EEC Activated sludge: Assessment of the respiration inhibition

Deviations:

no

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Source: activated sludge from laboratory wastewater treatment plant treating municipal sewage (Husmann 1-3 (8:2))
- Initial biomass concentration: 5 g/L dry matter in stock vessel; 1 g/L dry matter in test assay

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test temperature:

Ambient temperature

Nominal and measured concentrations:

Nominal: 10, 100, 1000 mg/L

Details on test conditions:

Test system:
- Test vessel: no further details
- Material, size, headspace, fill volume:
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

Effect parameters observed (with observation intervals if applicable) :
- Respiration: 30 min

Test concentrations:
- Spacing factor for test concentrations: 10

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Key result

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: EC20 = 8 mg/L; EC50 = about 18 mg/L

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the nominal 30 min EC10 of the substance in activated sludge was determined to be >1000 mg/L.

Executive summary:

A study was conducted to determine the toxicity of the read-across substance 2,2’-iminodiethanol (diethanolamine) to microorganisms according to OECD Guideline 209 and EC Directive 88/302/EEC. Activated sludge of a predominantly domestic sewage was exposed to the test substance at nominal concentrations of 10, 100 and 1000 mg/L for 30 minutes. A positive control (3,5-dichlorophenol) was included in the experiment. The inhibition of total respiration was monitored throughout the study. Under the study conditions, the nominal 30 min EC10 of the substance in activated sludge was determined to be >1000 mg/L (BASF AG, 1992).

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Read across study

Justification for type of information:

Refer to the section 13 of the IUCLID dataset for details on the read across justification. The toxicity to microorganisms study with the read across substance is considered sufficient to fulfil the information requirements.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Qualifier:

according to guideline

Guideline:

EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)

Deviations:

yes

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Up to 1 g/L cloudy solution

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Activated sludge from the municipal STP Hildesheim

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test temperature:

21°C

Nominal and measured concentrations:

0, 100, 180, 320, 580 and 1000 mg/L (nominal) + control

Reference substance (positive control):

yes

Remarks:

Copper(II)sulphate-pentahydrate.

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Results with reference substance (positive control):

The positive control gave a 3h EC50 respiration inhibition of 47 and 55%.

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the nominal 3 h EC50 of the substance in activated sludge was determined to be >1000 mg/L.

Executive summary:

A study was conducted to determine the toxicity of the read-across substance Phosphoric acid, dodecyl ester, potassium salt (mono- C12 PSE, K+) to microorganisms in activated sludge according to OECD Guideline 209 and EU Method C.11, in compliance with GLP. Activated sludge from a domestic sewage treatment plant was exposed to an aqueous dispersion of the test substance at nominal concentrations of 0, 100, 180, 320, 280 and 1000 mg/L. The rate of respiration was determined after 3 h contact and compared to data for the control. Copper(II)sulphate-pentahydrate was used as positive control and gave a 3 h EC50 (respiration inhibition of 47 and 55%). The 3 h EC20 and EC80 for the test substance were greater than 1000 mg/L. Under the study conditions, the nominal 3 h EC50 of the substance in activated sludge was determined to be >1000 mg/L (Noack, 1991).

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

EC10 or NOEC for microorganisms:

1 000 mg/L

Additional information

A study was conducted to determine the toxicity of the read-across substance Phosphoric acid, dodecyl ester, potassium salt (mono- C12 PSE, K+) to microorganisms in activated sludge according to OECD Guideline 209 and EU Method C.11, in compliance with GLP. Activated sludge from a domestic sewage treatment plant was exposed to an aqueous dispersion of the test substance at nominal concentrations of 0, 100, 180, 320, 280 and 1000 mg/L. The rate of respiration was determined after 3 h contact and compared to data for the control. Copper(II)sulphate-pentahydrate was used as positive control and gave a 3 h EC50 (respiration inhibition of 47 and 55%). The 3 h EC20 and EC80 for the test substance were greater than 1000 mg/L. Under the study conditions, the nominal 3 h EC50 of the substance in activated sludge was determined to be >1000 mg/L (Noack, 1991).

A study was conducted to determine the toxicity of the read-across substance 2,2’-iminodiethanol (diethanolamine) to microorganisms according to OECD Guideline 209 and EC Directive 88/302/EEC. Activated sludge of a predominantly domestic sewage was exposed to the test substance at nominal concentrations of 10, 100 and 1000 mg/L for 30 minutes. A positive control (3,5-dichlorophenol) was included in the experiment. The inhibition of total respiration was monitored throughout the study. Under the study conditions, the nominal 30 min EC10 of the substance in activated sludge was determined to be >1000 mg/L (BASF AG, 1992).