Absolute of Boswellia Carterii (Burseraceae) obtained from exudate by hexane treatment and subsequent ethanol extraction

Boiling point

Link to relevant study record(s)

Reference

Endpoint:

boiling point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07-14 February 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The test substance is adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 103 (Boiling Point)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.2 (Boiling Temperature)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

Inspected on 13 and 14 October 2014/ signed on 8 april 2015

Type of method:

method according to Siwoloboff

Key result

Boiling pt.:

193 °C

Atm. press.:

>= 99 340 - <= 99 872 Pa

Decomposition:

no

Findings:

Pre-test:

The test tube with test item was set into the aluminium block at 18.3 °C. The aluminium block was heated with gas burner.

The following observations were noted:

Observations	Temperatures
Shrivelled together and getting clearer	Since 50 °C
Completely melted to a yellow clear sticky mass with lots of fine foam	60 °C
Colourless condensate, foam slowly vanish	Since 120 °C
Some bubbles in large interval	Since 130 °C
Bubble chain, gas burner switched off, colour slightly darker	210 °C
Boiling chain broke	188.4 °C

After cooling down the test item appeared as a yellow-brown clear solid.

Main test:

Observations and measured values are presented in the following table:

Observations	Temperatures
	First Determination atmospheric pressure 99340 Pa	Second Determination atmospheric pressure 99872 Pa
Test start: Test item melted to a clear yellow viscous liquid with lots of fine foam.The foam vanishes slowly. Colourless condensate visible.	120.0 °C
No foam anymore.	Since 125 °C
Some bubbles in large interval	Since 150 °C	Since 129 °C
Boiling chain, gas burner switched off, colour slightly darker	225.9 °C	221 °C
Boiling chain broke	192.1 °C	193.9 °C

After cooling down the test item appeared as a yellow-brown clear solid.

No calculation following Sydney-Young was carried out as signs of decomposition (change of colour) were considered.

Results:

	Boiling point under decomposition	Pressure in Pa
First determination	192.1 °C (465.3 K)	99340 Pa
Second determination	193.9 °C (467.1 K)	99872 Pa
Mean	193.0 °C (466.2 K)

Mean ± standard deviation (without correction following Sydney Young)= 193.0 ± 1.3 °C (466.2 ± 1.3 K)

Conclusions:

The determination of the boiling point of test item was performed following the Siwoloboff Method.
First signs of possibly decomposition indicated by a colourless condensate were observed since 120°C.
Therefore the boiling point of test item should be stated as: 193.0 ± 1.3 °C (466.2 ± 1.3 K) under decomposition of the test item.
This value is the mean of two independent determinations without Sydney-Young correction.

Executive summary:

The study was performed in order to determine the boiling point of test item according to OECD 103, resp. EU A.2, guideline, using the Siwoloboff method.

The boiling point of test item should be stated as: 193.0 ± 1.3 °C (466.2 ± 1.3 K) under decomposition of the test item.

Description of key information

The boiling point of test item should be stated as: 193.0 ± 1.3 °C (466.2 ± 1.3 K) under decomposition of the test item.

Key value for chemical safety assessment

Boiling point at 101 325 Pa:

193 °C

Additional information

A fully reliable experimental study, conducted according to OECD 103/ EU A2 guideline is available.

It is considered as a key study, and the result is retained as key data.