Fatty acids, C16-18 (even numbered), reaction products with diethylene triamine, di-Me sulfate quaternized

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Qualifier:

according to guideline

Guideline:

other: DIN 38 412 Teil 25 (Statischer Test), CSB-Bestimmung nach DIN 38 409 H 41

Deviations:

no

Principles of method if other than guideline:

- Principle of test: Measurement of oxidative degradation of organic substances using the COD-method.
- Short description of test conditions: Test-vessels were prepared with 200 mL of dissolved substance (Stock-solution: 2.4 g of the test item dissolved in 1000 mL water), 600 mL dilution water, 50 mL inoculum and 2.5 mL growth-medium and incubated for 28 days under anaerobic conditions. The inoculum was filtered before use and as positive control sodium benzoate was used.
- Parameters analysed / observed:

GLP compliance:

no

Oxygen conditions:

anaerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Schlammgemisch aus den Kläranlagen Bonn, Köln, Siegburg, Berg.-Gladbach
- Preparation of inoculum for exposure: inoculum was filtered
- Concentration of sludge: 1.2 g/L
- Type and size of filter used, if any: Filter with 0.2 µm pore size

Duration of test (contact time):

28 d

Initial conc.:

480 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

other: COD elimination

Details on study design:

CONTROL AND BLANK SYSTEM
- Inoculum blank: not specified
- Abiotic sterile control: not specified
- Toxicity control: Measurement of Dehydrogenase activity with 2,3,5 - Triphenyltetrazoliumchlorid (TTC)

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

other: COD Elimination

Value:

25

Sampling time:

1 d

Key result

Parameter:

other: COD Elimination

Value:

50

Sampling time:

5 d

Key result

Parameter:

other: COD Elimination

Value:

65

Sampling time:

10 d

Key result

Parameter:

other: COD Elimination

Value:

> 90

Sampling time:

28 d

Results with reference substance:

After 28 days sodium benzoate was degraded to > 95%.

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

The biodegradability of the test item Rewoquat W1599 was determined according to DIN 38 412 Teil 25 by COD-Elimination. After 28 days the test item was degraded up to 90%, at day 10 biodegradation of the substance was approx. 65%. Thus, under the present conditions Rewoquat W1599 was readily biodegradable.

Executive summary:

In a study conducted according to DIN 380412 Teil 25, the test item Rewoquat W1599 was tested for biodegradability by COD-Elimination. 2.4 g test item/L were incubated with 50 mL inoculum from a local sewage traetment plant for 28 days. Over 90% of the substance was degraded after 28 days, thus, the substance was considered to be readily biodegradable.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Version / remarks:

1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Version / remarks:

2008

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Kläranlage, Sunthelle 6, 57392 Schmallenberg, Germany
- Storage conditions: kept aerobic and filtered through a coarse filter paper.
- Storage length: until use in the test, at least 1 h
- Concentration of sludge: 1 mL/L

Duration of test (contact time):

28 d

Initial conc.:

20 mg/L

Based on:

test mat.

Remarks:

test suspension A

Initial conc.:

40 mg/L

Based on:

test mat.

Remarks:

test suspension B

Initial conc.:

2.83 mg/L

Based on:

ThOD

Remarks:

test suspension A

Initial conc.:

5.66 mg/L

Based on:

ThOD

Remarks:

test suspension B

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: ( a ) KH2PO4 8.50 g/L
K2HPO4 21.75 g/L
Na2HPO4 26.64 g/L
NH4Cl 0.51 g/L
( b ) CaCl2 x 2H2O 36.41 g/L
( c ) MgSO4 x 7H2O 22.50 g/L
( d ) FeCl3 x 6H2O 0.25 g/L.
The mineral medium applied in the test contained 1 mL/L of the mineral stock solution a–d, respectively. The mineral medium was strongly aerated for 20 minutes and allowed to stand for 20 h at test temperature.
- Test temperature: 20 °C (± 1 °C)
- Aeration of dilution water: yes
- Continuous darkness: yes

SAMPLING
- Sampling frequency: day 3*, 6, 10*, 14, 21*, and 28 (* without toxicity control) using an O2 electrode.

CONTROL AND BLANK SYSTEM
- Inoculum blank: 16 vessels containing only inoculum
- Toxicity control: 8 vessels containing test item (40 mg/L), reference item (2 mg/L) and inoculum

STATISTICAL METHODS:
Theoretical Oxygen Demand (ThOD):
The Theoretical Oxygen Demand (ThOD) was calculated on the basis of the sum formula of the test and reference item considering complete nitrification.
Biochemical Oxygen Demand (BOD):
The Biochemical Oxygen Demand (BOD) was calculated on the basis of the test raw data by BOD [mg/mg] = mg O2 uptake corrected by blank per mg test item.
The percent degradation was calculated according to the following formula:
D t = [(C t –C b ) / ThOD] x 100
D t : degradation (%) at time t;
C t : mean oxygen consumption (mg/L) in the test suspension at time t;
C b : mean oxygen consumption (mg/L) in the blanks at time t;
ThOD: Theoretical oxygen demand of the test suspension (mg/L).

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (O2 consumption)

Value:

3.7

St. dev.:

1.2

Sampling time:

14 d

Remarks on result:

other: test suspension A

Parameter:

% degradation (O2 consumption)

Value:

1.7

St. dev.:

0.7

Sampling time:

14 d

Remarks on result:

other: test suspension B

Parameter:

% degradation (O2 consumption)

Value:

96.9

St. dev.:

1

Sampling time:

14 d

Remarks on result:

other: Procedural control

Parameter:

% degradation (O2 consumption)

Value:

28

St. dev.:

4.1

Sampling time:

14 d

Remarks on result:

other: Toxicity control

Parameter:

% degradation (O2 consumption)

Value:

3.4

St. dev.:

2

Sampling time:

28 d

Remarks on result:

other: test suspension A

Parameter:

% degradation (O2 consumption)

Value:

-1.2

St. dev.:

0.6

Sampling time:

28 d

Remarks on result:

other: test suspension B

Parameter:

% degradation (O2 consumption)

Value:

87.4

St. dev.:

2.3

Sampling time:

28 d

Remarks on result:

other: Procedural control

Parameter:

% degradation (O2 consumption)

Value:

26

St. dev.:

1.7

Sampling time:

28 d

Remarks on result:

other: Toxicity control

Results with reference substance:

The biodegradation of sodium benzoate was 87.4% after 28 days.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test item hydrogenated tallow/nortallow based IQAC, aqueous dispersion (5 %) must be considered as not readily biodegradable under the chosen test conditions.

Executive summary:

The biodegradation of hydrogenated tallow/nortallow based IQAC, aqueous dispersion (5% ) was investigated over a 28-day period in a Closed Bottle Test according to OECD guideline 301 D (1992). The test medium was inoculated with activated sludge from a sewage treatment plant mainly fed with municipal wastewater. The rate of degradation was monitored by measuring the oxygen consumption over the 28-d period.

The test item was tested with a concentration of 20 and 40 mg/L in duplicates, corresponding to a carbon content (ThOC) of 2.83 and 5.66 mg C/L in the test vessels.

Degradation is followed by analysis of dissolved oxygen over a 28-day period. The amount of oxygen taken up by the microbial population during biodegradation of the test item (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of ThOD.

The 10 % level (beginning of biodegradation of the test item) was reached within an adaptation phase of 3 days for the procedural control. The percentage of degradation of the functional control reached the pass level of 60 % after 3 days.

The highest mean biodegradation rate of hydrogenated tallow/nortallow based IQAC aqueous dispersion (5 %) within the 28 days of incubation in the static test was found to be 6 % (day 6) in the assays with 20 mg/L and 3 % (day 21) in the assays with 40 mg/L. Thus, no biodegradation within a 10-day-window could be obtained. The biodegradation of the item mixture in the toxicity control was found to be 28 % after 14 days of incubation. Thus, the demanded threshold value of 25 % is exceeded and the test item IS-2017-761 aqueous dispersion (5 %) can be identified as non-toxic in a ready biodegradability test. The reference item sodium benzoate was degraded to 97 % within the first 14 days, which was the highest degradation rate found within the test duration. In conclusion, according to the guideline, the test item hydrogenated tallow/nortallow based IQAC aqueous dispersion (5 %) must be identified as not readily biodegradable under the chosen test conditions.

Description of key information

-study conducted according to DIN 98 412 Teil 25, 1987, non-GLP, 2.4 g test item/L were incubated with 50 mL inoculum from a local sewage traetment plant for 28 days. Over 90% of the substance was degraded after 28 days

thus, the substance was considered to be readily biodegradable, supporting study

- study conducted according to OECD guideline 301 D, Closed Bottle Test, 1992, GLP, activated sludge from a sewage treatment plant mainly fed with municipal wastewater was tested with a concentration of 20 and 40 mg/L in duplicates, corresponding to a carbon content (ThOC) of 2.83 and 5.66 mg C/L in the test vessels. The rate of degradation was monitored by measuring the oxygen consumption over the 28-d period, no significant biodegradation occurred during the 28 days test period

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

The biodegradation of hydrogenated tallow/nortallow based IQAC (5% aqueous dispersion) was investigated over a 28-day period in a Closed Bottle Test according to OECD guideline 301 D (1992). The test medium was inoculated with activated sludge from a sewage treatment plant mainly fed with municipal wastewater. The rate of degradation was monitored by measuring the oxygen consumption over the 28-d period.

The test item was tested with a concentration of 20 and 40 mg/L in duplicates, corresponding to a carbon content (ThOC) of 2.83 and 5.66 mg C/L in the test vessels.

Degradation is followed by analysis of dissolved oxygen over a 28-day period. The amount of oxygen taken up by the microbial population during biodegradation of the test item (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of ThOD.

The 10 % level (beginning of biodegradation of the test item) was reached within an adaptation phase of 3 days for the procedural control. The percentage of degradation of the functional control reached the pass level of 60 % after 3 days.

The highest mean biodegradation rate of hydrogenated tallow/nortallow based IQAC aqueous dispersion (5 %) within the 28 days of incubation in the static test was found to be 6 % (day 6) in the assays with 20 mg/L and 3 % (day 21) in the assays with 40 mg/L. Thus, no biodegradation within a 10-day-window could be obtained. The biodegradation of the item mixture in the toxicity control was found to be 28 % after 14 days of incubation. Thus, the demanded threshold value of 25 % is exceeded and the test item hydrogenated tallow/nortallow based IQAC aqueous dispersion (5 %) can be identified as non-toxic in a ready biodegradability test. The reference item sodium benzoate was degraded to 97 % within the first 14 days, which was the highest degradation rate found within the test duration. In conclusion, according to the guideline, the test item hydrogenated tallow/nortallow based IQAC aqueous dispersion (5 %) must be identified as not readily biodegradable under the chosen test conditions.

Additionally, in a study conducted according to DIN 380412 Teil 25, the test item Rewoquat W1599 was tested for biodegradability by COD-Elimination. 2.4 g test item/L were incubated with 50 mL inoculum from a local sewage traetment plant for 28 days. Over 90% of the substance was degraded after 28 days, thus, the substance was considered to be readily biodegradable.

Based on the available information hydrogenated tallow/nortallow based IQAC is considered to be not readily biodegradable.

[Type of water: freshwater]