Sodium 3-sulphonatopropyl acrylate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 Aug - 17 Aug 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP - Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Remarks:

The Department of Health of the Government of the United Kingdom

Test material

Test material information

Constituent 1

Reference substance name:

Potassium 3-sulphonatopropyl acrylate

EC Number:

250-465-0

EC Name:

Potassium 3-sulphonatopropyl acrylate

Cas Number:

31098-20-1

Molecular formula:

C6H10O5S.K

IUPAC Name:

potassium 3-(acryloyloxy)propane-1-sulfonate

Details on test material:

- Name of test material (as cited in study report): SPA (sulfopropyl acrylate)
- Physical state: powder
- Analytical purity: no data
- Lot/batch No.: 0200014/001
- Storage condition of test material: at room temperature in the dark

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Pooled replicate water samples were taken from the control and the 100 mg/L test group at 0 and 48 h for quantitative analysis.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 200 mg test material was directly added to reconstituted water (ISO medium) and the volume adjusted to give a test concentration of 100 mg/L. The solution was inverted several times to ensure adequate mixing and homogeneity.
- Evidence of undissolved material: The test preparations were observed to be clear, colourless solutions throughout the study.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: in-house culture
- Age at study initiation: < 24 h
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

21 °C, measured at test start and after 24 and 48 h in every test vessel and the control.

pH:

7.9 - 8.0, measured at test start and after 48 h in every test vessel and the control.

Dissolved oxygen:

8.1 - 8.4 mg O2/L, (91 - 94% of saturation), measured at test start and after 48 h in every test vessel and the control.

Nominal and measured concentrations:

nominal: 0 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel
- Type: Test vessels were covered with glass lids to reduce evaporation.
- Material, size, fill volume: 250 mL glass jar containing approx. 200 mL test solution
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (ISO medium) as stated in OECD 202

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark with 20 min dawn and dusk transition periods

EFFECT PARAMETERS MEASURED: immobilisation and adverse reactions after 24 and 48 h

TEST CONCENTRATIONS
- Range finding study:
- Test concentrations: 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: No immobilisation was observed up to a concentration of 100 mg/L.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: No adverse reactions were observed.
- Mortality of control: no

Any other information on results incl. tables

Analysis of the test preparations sampled at test start and after 48 h showed the measured test concentrations to be near nominal (102 - 105%).