3-[[9,10-dihydro-9,10-dioxo-4-[(p-tolyl)amino]-1-anthryl]aminopropyl]trimethylammonium methyl sulphate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 Feb - 02 Mar 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Benzyl[3-[[9,10-dihydro-4-[isopropylamino]-9,10-dioxo-1-anthryl]amino]propyl]dimethylammonium chloride

EC Number:

252-547-1

EC Name:

Benzyl[3-[[9,10-dihydro-4-[isopropylamino]-9,10-dioxo-1-anthryl]amino]propyl]dimethylammonium chloride

Cas Number:

35402-38-1

Molecular formula:

C29H34N3O2.Cl

IUPAC Name:

N-benzyl-3-{[4-(isopropylamino)-9,10-dioxo-9,10-dihydroanthracen-1-yl]amino}-N,N-dimethylpropan-1-aminium chloride

Test material form:

solid: particulate/powder

Details on test material:

Basic Blue 123 Cl

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all
- Sampling method: taken from approximate centre of test vessel
- Sample storage conditions before analysis: kept at -18°C to -25°C until analysis

Test solutions

Vehicle:

yes

Remarks:

Tween 80

Details on test solutions:

0.5 g test substance were mixed with 200 mL test medium in a beaker
mixture was homogenized for 8 minutes by ultrasonic treatment and filled up to 500 mL with test medium

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: own breed
- Feeding during test: no
- Food type: suspension of green algae and Tetramin extract
- Amount: amount needed for 24 h
- Frequency: daily

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

231 mg/L CaCO3 (test medium)

Test temperature:

21°C

pH:

7.8 - 8.0

Dissolved oxygen:

97 - 99% saturation

Nominal and measured concentrations:

Nominal: 0 (control), 0 (solvent control), 4.3, 9.4, 21, 45 and 100 mg/L
Measured (arithmetic mean):

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker
- Material, size, headspace, fill volume: 250 mL, fill volume: 60-100 mL
- Aeration: no
- No. of organisms per vessel: 1ß
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Aqua bidist.
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Culture medium different from test medium: no
- Intervals of water quality measurement: beginning of each test

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12 h

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility (24, 48h)


RANGE-FINDING STUDY
- Test concentrations: 0.01 - 100 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

8.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

4.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no
- Mortality of control / solvent control: no

Results with reference substance (positive control):

24-h EC50: 0.95 mg/L corresponding to a normal sensitivity (25 Jan 1995)

Reported statistics and error estimates:

Probit model

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-h EC50 to Daphnia magna was determined at 8.3 mg/L based on nominal concentrations (analytically verified). The substance is acutely harmful to aquatic invertebrates.