Sodium methyl sulphate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017-05-16 - 2017-05-18

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Sodium methyl sulphate

EC Number:

208-142-7

EC Name:

Sodium methyl sulphate

Cas Number:

512-42-5

Molecular formula:

CH4O4S.Na

IUPAC Name:

sodium methyl sulphate

Test material form:

solid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: KIRSCHAZ2-00182
- Expiration date of the lot/batch: 2018-07-07
- Water solubility: 925 g/L ± 9 g/L at T = 20.0 °C ± 0.5 °C

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature, protect from light, in the tightly closed original container
- Stability under test conditions: stable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 120 mg/L
- Sampling method: Before the start of the exposure and at renewal (0 and 24 hours), samples of the fresh media were taken after preparation of the limit concentration and analyzed. At water renewal and at the end of the exposure (24 and 48 hours), samples of the old media were taken directly from the test vessels for analyses
- Sample storage conditions before analysis: All original samples were stored at room temperature. Prepared samples were stored at room temperature in an autosampler until analysis

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- The limit concentration (120 mg/L were weighed out) was freshly prepared with dilution water prior to the start of the exposure (0 hours) and before media renewal (24 hours).
The limit concentration was mixed thoroughly by manual agitation. No Tyndall effect was observed in the limit concentration before insertion of the daphnids.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: (Clone 5)
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
- Breeder: Noack Laboratorien GmbH, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany
- Age of parental stock (mean and range, SD): Less than 24 hours old daphnids from a healthy stock were used for the study. Juvenile daphnids were removed from the culture vessels at the latest 24 hours before the start of the exposure and discarded. The juveniles born within the following period of max. 24 hours preceding the exposure were used for the test. No first brood progeny was used for the test.
- Feeding during test: The daphnids were not fed during the study.


ACCLIMATION
- The culture daphnids were fed at least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella
subcapitata and Desmodesmus subspicatus, with an algae cell density of > 106 cells/mL. The algae were cultured at the test facility.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

163 - 173 mg CaCO3/L

Test temperature:

20.6 - 20.7 °C

pH:

7.85 - 8.38

Dissolved oxygen:

7.64 - 8.17

Conductivity:

460-480 μS/cm

Nominal and measured concentrations:

nominal: 120 mg/L; measured 132 - 133 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity, loosely covered with watch glasses
- Test volume: 20 mL
- Renewal rate of test solution (frequency/flow rate): The test solutions were renewed after 24 hours. For this purpose, a second set of test vessels was filled with the freshly prepared test
solutions and the daphnids were transferred by pipette.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 202

OTHER TEST CONDITIONS

- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: Diffuse light, light intensity of max. 1500 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
lmmobilization was determined in all groups after 24 and 48 hours. An animal was considered immobile, if it was not able to swim in the water phase within 15 seconds after gentle agitation of the test
vessel.


Non-GLP Preliminary Range Finding Test
- Test concentrations: 0, 1.0, 10.0 and 100 mg/L.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance:
EC50-Value of the Reference ltem Potassium dichromate based on nominal concentrations [mg/L], (0 - 24 hours): 1.60 mg/L
95% confidence limits: 1.43 - 1.79 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

At the limit concentration of 120 mg/L of the test item Sodium methyl sulfate, no effects on Daphnia magna STRAUS were observed.