2-(oxolan-2-ylmethoxy)ethanol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 - 22 Jul 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

2008

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Hessisches Ministerium für Umwelt, Klimaschautz, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany

Test material

Test material information

Constituent 1

Reference substance name:

2-[(tetrahydrofurfuryl)oxy]ethanol

EC Number:

227-407-8

EC Name:

2-[(tetrahydrofurfuryl)oxy]ethanol

Cas Number:

5831-59-4

Molecular formula:

C7H14O3

IUPAC Name:

2-(tetrahydrofuran-2-ylmethoxy)ethanol

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all test item concentrations and the control
- Sampling method: Samples were taken at test start and test end, duplicate samples were taken at test start and test end.
- Sample storage conditions before analysis: All samples were stored in a refrigerator (4 ± 4 °C), protected from light until analysis was performed.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The limit test concentration of 100 mg/L was prepared by dissolving 77.1 mg test item into 771 mL test water by intense stirring for 10 min in the dark.
- Eluate: no
- Differential loading: yes
- Appearance of test solutions: Test solutions were clear and colorless.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Female Daphnia magna, clone 5
- Source: in-house laboratory culture
- Feeding during test: no only stock culture
- Food type: Desmodesmus subspicatus
- Frequency: at least on all working days
- Age: < 24 h

ACCLIMATION
- Acclimation conditions: same
- Type and amount of food: Desmodesmus subspicatus
- Frequency: at least on all working days

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: The test organisms were not first brood progeny.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/L (= 250 mg/L) as CaCO3

Test temperature:

20.4 to 20.6 °C

pH:

7.9 to 8.0

Dissolved oxygen:

8.3 to 8.7 mg/L

Conductivity:

< 5 µS/cm

Nominal and measured concentrations:

Control and 100 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
Test vessel:
- Type: open (covered with a lid)
- Material, size, headspace, fill volume: glass, 100 mL, headspace: 20 mL, fill volume: 80 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium according to the guideline OECD 202
- Alkalinity: 0.9 mmol/L
- Conductivity: < 5 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: every 24 h

OTHER TEST CONDITIONS
- Photoperiod: 16 h light - 8 h dark
- Light intensity: 500 to 640 lux

EFFECT PARAMETERS MEASURED: The mobility of the daphnids was determined by visual observation after 24 and 48 h. Those animals that are not able to swim within 15 sec after gentle agitation of the test beaker were considered to be immobile (even if they could still move their antennae).

RANGE-FINDING STUDY: non-GLP range-finding test

Reference substance (positive control):

yes

Remarks:

Potassium dichromate, at least twice a year

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no abnormal behaviour
- Mortality of control: no mortality

Results with reference substance (positive control):

Results of the most recent test with Potassium dichromate:
EC50 (48 h): 1.06 mg/L

Reported statistics and error estimates:

No statistical analysis was performed. The EC50 could not be quantified due to the absence of toxicity of the test item. The NOEC and the LOEC were determined directly from the raw data.

Any other information on results incl. tables

Analytical Results:

Table 1: Results for the Determination of the Test Item in the Test Samples

Sample Description		Total Cabon			Inorganic Carbon			Total Organic Carbon
	Age	found		calculated	found		calculated	TOC	corrected TOC 2)	nominal 3)	% of nominal
[mg test item/L]	[d]	[mg C/L] 1)	DF	[mg C/L] 1)	[mg C/L] 1)	DF	[mg C/L] 1)	[mg C/L]	[mg C/L]	[mg C/L]
Control	0	12.236	1	12.236	8.956	1	8.956	3.281	n.a.	0.000	n.a.
Control	0	12.182	1	12.182	8.875	1	8.875	3.307	n.a.	0.000	n.a.
Control	2	12.500	1	12.500	9.159	1	9.159	3.341	n.a.	0.000	n.a.
Control	2	12.185	1	12.185	9.163	1	9.163	3.022	n.a.	0.000	n.a.
100	0	77.910	1	77.910	8.894	1	8.894	69.016	65.778	63.543	104
100	0	77.983	1	77.983	8.908	1	8.908	69.075	65.837	63.543	104
100	2	76.828	1	76.828	9.159	1	9.159	67.669	64.431	63.543	101
100	2	77.099	1	77.099	9.126	1	9.126	67.973	64.735	63.543	102
mean value (n=4):											103
RSD (n=4):											1

1 The tabulated results represent rounded results calculated on the exact raw data

2 The results were corrected by the mean value of the control (carbon content of test media)

3 nominal Carbon content of the test item: 63.543 mg C/L in 100 mg test item/L (determined by measuring TOC content in pure water)

RSD: Relative Standard Deviation; DF: Dilution Factor

Biological Results:

Table 2: Endpoints: Influence of Glycofurol on the Mobility of Daphnia magna

Endpoint [mg test item/L]	24 hours	48 hours
EC50	> 100	> 100
95 % CI	n.d.	n.d.
EC20	> 100	> 100
95 % CI	n.d.	n.d.
EC10	> 100	> 100
95 % CI	n.d.	n.d.
NOEC	100	100
LOEC	> 100	> 100

Values refer to nominal test concentrations.
CI: Confidence interval
n.d.: not determinable
NOEC and LOEC were determined directly from the raw data.

Table 3: Validity criteria for OECD 202

Criterion from the guideline	Outcome	Validity criterion fulfilled
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.	0 %	Yes
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.	8.3 mg O2/L	Yes

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes