Bis(triethoxysilylpropyl)amine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16 - 20 Nov 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

following OSPAR PARCOM Protocol, marine fish, semi-static

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Control and nominal loading rate of 200 mg/L
- Sampling method: 4 mL samples of the test media were taken at the start and end of each successive 48 h exposure period (total exposure period of 96 h)
- Sample storage conditions before analysis: Samples analysed immediately after collection. Retained samples were stored at -20 °C

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test item was added to the test vessel containing dilution water and stirred for 1 hour prior to introducing the fish.
- Controls: Dilution water

Test organisms (species):

other: Scophthalmus maximus

Details on test organisms:

TEST ORGANISM
- Common name: Turbot
- Source: Venö Fish Farm, Venö, Denmark
- Diameter at study initiation (mean and SD): 5 +/-1 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: 15 days
- Acclimation conditions: same as test
- Type and amount of food: DANA Feed 1362, 1.8 mm granulate
- Feeding frequency: Twice per day for at least 5 days per week
- Health during acclimation (any mortality observed): No fish died during the acclimation period

Test type:

semi-static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

not reported

Test temperature:

14.2-14.7°C

pH:

7.8-8.6

Dissolved oxygen:

≥60% ASV

Salinity:

33.5 ppm

Nominal and measured concentrations:

Control and nominal loading rate of 200 mg/L
Mean measured concentrations of the test substance (based on DOC) were 113 and 118% of nominal in the two replicates.

Details on test conditions:

TEST SYSTEM
- Test vessel: aquarium

- Type: open

- Material, size, fill volume: glass, 10 litre with 7 L fill volume

- Aeration: yes

- Renewal rate of test solution: after 48 hours

- No. of organisms per vessel: 7

- No. of vessels per concentration (replicates): 2

- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Articial seawater prepared using "Reef Salt", Aqua Medic GmbH, Bissendorf, Germany by adding 33.5 g/L of salts to tap water and stirring for 24 hours.

- Ca/mg ratio: 1:2.9

- Culture medium different from test medium: no

- Intervals of water quality measurement: Daily

OTHER TEST CONDITIONS

- Adjustment of pH: no

- Photoperiod: 12 h light, 12 h dark

EFFECT PARAMETERS MEASURED: Mortality daily

TEST CONCENTRATIONS

- Spacing factor for test concentrations: Limit test

- Range finding study

- Test concentrations: 1, 10, 50, 100, 200, 500 and 1000 mg/L

- Results used to determine the conditions for the definitive study: No mortality at any concentration

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: 0%

Reported statistics and error estimates:

There were no toxic effects observed in the test and therefore statistical analysis of the results was not required.

Sublethal observations / clinical signs:

Table 1. Results of analysis of test media

 

Nominal test substance concentration (mg/L)	Nominal test substance concentration as TOC (mg/L)	Measured DOC concentration in fresh test medium at start of test (mg/L)	Measured DOC concentration in old test medium after 48 hours (mg/L)	Measured DOC concentration in fresh test medium at 48 hours (mg/L)	Measured DOC concentration in old test medium after 96 hours (mg/L)
0 (Control)	0	8.2	20	18	3.7
200 (Rep. 1)	84	115	97	97	84
200 (Rep. 2)	84	100	97	97	84

 

Since no toxic effects were observed up to the highest tested concentration the NOEC (96 h) was >= 200 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

A 96-h LC50 value of >200 mg/L and a NOEC of ≥200 mg/L have been determined for the effects of the test substance on mortality of the marine fish species Scophthalmus maximus based on nominal exposure concentrations.

Description of key information

LC50 (96 h) > 200 mg/L (nominal, Scophthalmus maximus, OECD 203)

Key value for chemical safety assessment

Marine water fish

Marine water fish

Dose descriptor:

LC50

Effect concentration:

> 200 mg/L

Additional information

Experimental data are available testing the acute toxicity of 3-(triethoxysilyl)-N-[3-(triethoxysilyl)propyl]-1-propanamine (CAS No. 13497-18-2) towards fish. The study was performed according to the OECD guideline 203 and GLP standards (2004). The salt water organism Scophthalmus maximus was used for testing and was exposed for 96 h in a semi-static testing regime to one single test item concentration of 200 mg/L and a control. The test item was monitored during the test via measurement of DOC. The recoveries of the test substance were 113 and 118% of the nominal concentration and therefore results refer to nominal values. No toxic effects were recorded and thus the study resulted into a 96-h LC50 value of > 200 mg/L and a NOEC of ≥ 200 mg/L (nominal).