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EC number: 270-821-9 | CAS number: 68478-46-6
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item in the present study. The test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany.
The test was carried out under static conditions with the test item concentrations 4 - 16 - 64 - 256 - 1000 mg/L in the presence or absence of the specific nitrification inhibitor ATU. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours. The inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of total respiration for the chosen test item replicates concentrations ranged from -8 % to 84 %. The mean inhibition of the heterotrophic respiration for the test item replicates ranged from -3 % to 71 %. The mean inhibition of nitrification for the test item replicates ranged from -14 % to 95 %.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate and N-Methylaniline (with and without ATU) as reference item and the reference toxicity was determined. The EC50-value for the reference item copper (II) sulphate pentahydrate was 96.3 mg/L. The EC50-value for the reference item N-Methylaniline was
2.47 mg/L.
In conclusion, the effect values of the test item in the respiration inhibitory test with activated sludge are as follow:
The NOEC for total respiration is 64 mg/L.
The EC10 for total respiration is 147 mg/L and the EC50 is 450 mg/L.
The NOEC for heterotrophic respiration is 64 mg/L.
The EC10 for heterotrophic respiration is 251 mg/L and the EC50 is 666 mg/L.
The NOEC for nitrification is 64 mg/L.
The EC10 for nitrification is 114 mg/L and the EC50 is 332 mg/L.
The validity criteria were fulfilled according to the guideline.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 450 mg/L
- EC10 or NOEC for microorganisms:
- 147 mg/L
Additional information
EC10 value of 147 mg/L with confidence interval of [133 -160] was preferred to NOEC value of 64 mg/L.
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