2,3-difluoro-1-propoxy-4-[4-(trans-4-propylcyclohexyl)-1-cyclohexen-1-yl]-benzene

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 16, 2013 - December 31, 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

1984

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.6200 (Earthworm Subchronic Toxicity Test)

Version / remarks:

1996

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,3-difluoro-1-propoxy-4-{4-[(1s,4r)-4-propylcyclohexyl]cyclohex-1-en-1-yl}benzene

EC Number:

600-065-0

Cas Number:

1003218-40-3

Molecular formula:

C24H34F2O

IUPAC Name:

2,3-difluoro-1-propoxy-4-{4-[(1s,4r)-4-propylcyclohexyl]cyclohex-1-en-1-yl}benzene

Test substrate

Details on preparation and application of test substrate:

- Method of mixing into soil: directly added

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

nematods

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm
- Source: Nanjing Earthworm Nursery, China
- Age at test initiation (mean and range, SD): adult
- Weight at test initiation: 394 - 400 mg

ACCLIMATION
- Acclimation period: 94 days

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test conditions

Test temperature:

20 - 22°C

pH:

6.0 - 6.5

Moisture:

35%

Details on test conditions:

TEST SYSTEM
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 400 - 800 lux

EFFECT PARAMETERS MEASURED:
mortality, reaction on mechanical stimulus, behavioural and pathological symptoms assessed at days 7 and 14

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 10, 100, 1000 mg/kg dw

Nominal and measured concentrations:

Nominal concentration 1000 mg/kg dw

Reference substance (positive control):

yes

Remarks:

2-Chloroacetamide, purity >=98.5%

Results and discussion

Effect concentrations

Key result

Duration:

14 d

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Any other information on results incl. tables

Study design

In this GLP study performed according to OECD GL 207 the acute toxicity of the test item in earthworms was investigated. During this test, the artificial soils was used with the moisture content of the test medium controlled in range of 30 - 36 % with pH values of 6 - 6.5. The test conditions were maintained at 20 - 22°C, a humidity of 80 - 85 % and continuous light with intensity of 400 - 800 lux.

Results

No dead earthworms were observed in treatments and control. During test all animals remained in soil. Based on the result of range-finding test and the Limit Test, the 14 d-LC50 of the test item in the artificial soil was determined to be greater than 1000 mg/kg dry weight soil. The highest concentration causing no mortality (14 d-LC0) was 1000 mg/kg dry weight soil.

Conclusion

No mortality was observed within the test period. Therefore, the 14 d-LC50 was determined to be >1000 mg/kg dw.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A limit test on the acute toxicity of the test item was conducted according to OECD 207. The earthworm Eisenia fetida was used for the test in artificial soil. No mortality was observed within the test period. Therefore, the 14 d-LC50 was determined to be >1000 mg/kg dw.