Amides, vegetable-oil, N,N-bis(hydroxyethyl)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

Read across approach adequate to support 7.6.1 according to "Guidance on information requirements and chemical safety assessment Chapter R.6: QSARs and grouping of chemicals" (ECHA 2008) and Read-Across Assessment
Framework (RAAF) (ECHA 2017)

Cross-reference

Reason / purpose for cross-reference:

read-across source

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Read across approach adequate to support 7.6.1 according to "Guidance on information requirements and chemical safety assessment Chapter R.6: QSARs and grouping of chemicals" (ECHA 2008) and Read-Across Assessment
Framework (RAAF) (ECHA 2017)

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

not specified

Details on test solutions:

The test substancehas a water solubility of ≤ 4 mg/L. In view of the difficulties associated with the evaluation of aquatic toxicity of poorly water soluble test items, a modification of the standard method for the preparation of aqueous media was performed. An approach endorsed by several important regulatory authorities in the EU and elsewhere (ECETOC 1996 and OECD 2000), is to expose the organisms to a Water Accommodated Fraction (WAF) of the test item in cases where the test item is a complex mixture and is poorly soluble in water and in the permitted solvents.

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Water media type:

freshwater

Duration:

72 h

Dose descriptor:

NOELR

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Data source

Reference

Reference Type:

publication

Title:

UNNAMED

Author:

US EPA

Year:

1988

Bibliographic source:

HPVIS

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Reference substance 001

Cas Number:

68140-00-1

Test organisms

Test organisms (species):

other: Scenedesmus subspicatus

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Test conditions

Details on test conditions:

Algae exposed to a range of concentrations (three
replicates/concentration) in a mineral nutrient solution for
72 hours. Cell counts were made daily and treatment levels
were compared to the control group.

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

1.1 mg/L

Conc. based on:

test mat.

Basis for effect:

cell number

Remarks on result:

other: NES

Applicant's summary and conclusion

Conclusions:

EC50 > solubility limit for the substance, classified as insoluble/dispersible ( US EPA 2010): (NES)