Bis(pentane-2,4-dionato-O,O')zinc

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

GLP compliance:

not specified

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

5 animals for each test
4 replicates for each main test

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test temperature:

19 - 21 °C

pH:

7.5 - 8.1

Dissolved oxygen:

8.4 - 9.1 mg/L

Details on test conditions:

light/dark: 16/8 h

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

55 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Validity criteria fulfilled:

yes

Conclusions:

The NOEC and EC50 for Daphnia magna were determined to be 32 mg/L and 55 mg/L, respectively.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia were originally obtained from Wil Research B.V. Hambakenwetering 7, 5231 DD ‘s-Hertogenbosch.

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

12.3ºdH / CaCO3 concentration of 219 mg/L. The conductivity of DSW was measured as 593 µs/cm.

Test temperature:

21 - 25 °C

pH:

8.0

Dissolved oxygen:

9.1 - 9.2

Details on test conditions:

light/dark: 16/8 h
The test was performed as a static test for 48 hours. 20 animals divided into 4 batches of 5 animals were tested at each of 5 test concentrations and in the control.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

4.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Validity criteria fulfilled:

yes

Conclusions:

A NOEC of 2.0 mg/L and a LOEC of 4.5 mg/L were determined. The EC50 (48h) was calculated as 6.6 mg/L (Nominal) [Based on immobility].

Description of key information

Two studies with the test substance on Daphnia magna were conducted.

The NOEC and EC50 for Daphnia magna were determined to be 32 mg/L and 55 mg/L, respectively in the earlier study.

The NOEC and EC50 for Daphnia magna were determined to be 2.0 mg/L and 6.6 mg/L, respectively in the later study.

The later study was performed under GLP with a more detailed report.

Thus the later study is assumed to be more reliable and the following values were used for classification and assessment:

NOEC 48h: 2.0 mg/L

EC50: 6.6 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

6.6 mg/L