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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material: Reaction mass of Methanaminium, N-​[4-​[[4-​(dimethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​methyl-​ & acetate- Molecular formula: C25H28N2O2- Molecular weight: 388.508 g/mole- Smiles : [N+](=C1/C=CC(\C=C1)=C(\c1ccccc1)c1ccc(cc1)N(C)C)(\C)C.C(C)(=O)[O-]- Inchl: 1S/C23H25N2.C2H4O2/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;1-2(3)4/h5-17H,1-4H3;1H3,(H,3,4)/q+1;/p-1- Substance type: Organic- Physical state: maroon lustrous liquid-Batch No.: KDI/FS/13/18
Analytical monitoring:
yes
Remarks:
Stability was check with the help of spectrometer.
Vehicle:
no
Details on test solutions:
The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 mL of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 0.1562mg/L,0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM- Common name:Zebra Fish - Strain:Danio rerio- Source: Unique Aqua Fish Corner- Length at study initiation (length definition, mean, range and SD):average 3.42 cm- Weight at study initiation (mean and range, SD): average 0.335 g -Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.-Duration of housing: 15 days -Photoperiod: 12 hours light and 12 hours darkness-Diet : Standard brand feed, once daily-Acclimatization: seven days -feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
152.5 mg of CaCO3
Test temperature:
23°C
pH:
7.1
Dissolved oxygen:
7.0 mg/L
Nominal and measured concentrations:
0.1562mg/L,0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L
Details on test conditions:
TEST SYSTEM - Test vessel: plastic aquaria - volume: 2 liters - Aeration: Aeration in test vessels was provided 1 day before the start of experiment - No. of organisms per vessel: 8 fishes OTHER TEST CONDITIONS - Photoperiod:16 hours light and 8 hours dark
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.156 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:

8.1. Mortality and symptoms

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

0.1562

4

4

-

-

-

-

-

-

-

-

1

1

1

1

0.3125

4

4

-

-

-

-

-

-

-

-

1

1

2

2

0.625

4

4

-

-

-

-

-

-

4

4

4

4

4

4

1.25

4

4

-

-

-

-

4

4

4

4

4

4

4

4

10

4

4

-

-

-

-

4

4

4

4

4

4

4

4

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.1562

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.3125

4

4

NS4

NS4

NS4

NS4

NS4

NS4

-

-

-

-

-

-

0.625

4

4

NS4

NS4

NS4

NS4

-

-

-

-

-

-

-

-

1.25

4

4

NS4

NS4

NS4

NS4

-

-

-

-

-

-

-

-

2.5

4

4

NS4

NS4

NS4

NS4

-

-

-

-

-

-

-

 

Explanation of symptoms:

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

Nominal

Concentration

(mg/l)

Number of

fish

pH readings after

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

7.4

7.4

7.5

7.5

7.6

7.6

7.2

7.2

0.1562

4

4

7.8

7.8

7.9

7.9

8.0

8.0

8.1

8.1

0.3125

4

4

7.9

7.9

-

-

-

-

-

-

0.625

4

4

-

-

-

-

-

-

-

-

1.25

4

4

-

-

-

-

-

-

-

-

2.5

4

4

-

-

-

-

-

-

-

-

Nominal

Concentration

(mg/l)

Number of

fish

Oxygen content (mg/L) after

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

6.8

6.8

6.7

6.7

6.6

6.6

6.5

6.5

0.1562

4

4

6.7

6.7

6.6

6.6

6.5

6.5

6.3

6.3

0.3125

4

4

7.9

7.9

-

-

-

-

-

-

0.625

4

4

-

-

-

-

-

-

-

-

1.25

4

4

-

-

-

-

-

-

-

-

2.5

4

4

-

-

-

-

-

-

-

-

 

Nominal

Concentration

(mg/l)

Number of

fish

Temperature (°C) after

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

23.3

23.3

23.3

23.4

23.4

23.4

23.3

23.3

0.1562

4

4

23.3

23.3

23.4

23.4

23.4

23.4

23.3

23.3

0.3125

4

4

23.3

23.3

-

-

-

-

-

-

0.625

4

4

-

-

-

-

-

--

-

-

1.25

4

4

-

-

-

-

-

-

-

-

2.5

4

4

-

-

-

-

-

-

-

-

 

Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Reaction mass of Methanaminium, N-​[4-​[[4-​(dimethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​methyl-​ & acetate on Zebra Fish Danio rerio was found to be 0.1562 mg/L
Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Reaction mass of Methanaminium, N-​[4-​[[4-​(dimethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​methyl-​ & acetate on Zebra fish.

The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 mL of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 0.1562mg/L,0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,respectively. Stability was check with the help of spectrometer.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

The nominal concentration selected for the experiment was

0.1562mg/L,0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L and Zebra fish were exposed to this concentration for 96 hours. The lethal concentrations LC50 was found to be 0.1562 mg/L.

After 96 hours of exposure to test item Reaction mass of Methanaminium, N-​[4-​[[4-​(dimethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​methyl-​ & acetate to various nominal test concentrations, LC50 was determine to be 0.1562 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be aq aquatic chronic 1 as per the CLP classification criteria.

Description of key information

Short-term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted forReaction mass of Methanaminium, N-​[4-​[[4-​(dimethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​methyl-​ & acetateon Zebra fish.

The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 mL of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 0.1562mg/L,0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,respectively. Stability was check with the help of spectrometer.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

The nominal concentration selected for the experiment was

0.1562mg/L,0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/Land Zebra fish were exposed to this concentration for 96 hours. The lethal concentrations LC50 was found to be 0.1562 mg/L.

After 96 hours of exposure to test itemReaction mass of Methanaminium, N-​[4-​[[4-​(dimethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​methyl-​ & acetateto various nominal test concentrations, LC50 was determine to be 0.1562 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be aq aquatic chronic 1 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.156 mg/L

Additional information

Short-term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted forReaction mass of Methanaminium, N-​[4-​[[4-​(dimethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​methyl-​ & acetateon Zebra fish.

The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 mL of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 0.1562mg/L,0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,respectively. Stability was check with the help of spectrometer.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

The nominal concentration selected for the experiment was

0.1562mg/L,0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/Land Zebra fish were exposed to this concentration for 96 hours. The lethal concentrations LC50 was found to be 0.1562 mg/L.

After 96 hours of exposure to test itemReaction mass of Methanaminium, N-​[4-​[[4-​(dimethylamino)​phenyl]​phenylmethylene]​-​2,​5-​cyclohexadien-​1-​ylidene]​-​N-​methyl-​ & acetateto various nominal test concentrations, LC50 was determine to be 0.1562 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be aq aquatic chronic 1 as per the CLP classification criteria.