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Short-term toxicity to aquatic invertebrates

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Description of key information

EC50 (48h) > 100 mg/l

Key value for chemical safety assessment

Additional information

The aquatic toxicity against Daphnia magna of the substance was investigated using a specific QSAR model, developed to predict the acute oral toxicity for dyes. The existing QSAR models have strong limitations to predictionic complex structures as the organic dyes are, and consequently they provide unreliable results. The QSAR modelling was developed in accordance with the OECD principles (details in the documentationattached).

Based on the estimation, the substance is expected to have no concern foraquatic toxicity against Daphnia magna. The estimation resulted to be in the applicability domain of the model.

Thus, the substance can be considered as not classified foraquatic toxicity against Daphnia magna.

 

In order to confirm the results obtained by the QSAR prediction, the investigations performed on an analogue substance (Similar Substance 01) are used as support of the prediction. Justification for Read Across is given in Section 13 of IUCLID.

Acute toxicity to Daphnia magna was determined following OECD guideline 202. Toxic effects were evaluated in terms of mobility, using nominal test concentrations of 95, 171, 309, 556 and 1000 mg test substance /l and a control. Actual concentrations in test media were also measured.

In test media, measured concentrations of test substance only amounted to 29.2 - 41.7 % of nominal values. These low recovery rates were probably due to precipitation of test substance, which was seen in test media of all test concentrations during test duration and also in samples taken for analytical measurements. Since measured concentration of stock solution was 102.4 % of nominal value, results were reported as nominal concentrations of test substance.

Up to the highest concentration tested of 1000 mg test substance/l, no toxic effect on Daphnia magna was observed. Therefore, after 48 hours of exposure:

EC0 = 1000 mg/l and EC50 > 1000 mg/l.