Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14.03.2018 to 22.03.2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate

EC Number:

261-118-8

EC Name:

Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate

Cas Number:

58096-47-2

Molecular formula:

C16H26O2

IUPAC Name:

decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

In the range finding test, Neonates of daphnia were tested for immobility in test concentrations of Clovanyl-3-Formate (CARYOLAN) at 0.01, 0.1, 1, 10 and 100 mg/L along with a negative control.

In the definitive test, Neonates of daphnia were tested for immobility in test concentrations of 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L (factor of 2.2)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

One day prior to the start of the treatment, about 100 daphnia during range finding test and definitive test were incubated in approximately 2 L of test medium. The following day, the young ones hatched which were aged less than 24 hour old were collected and used in the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.39 mmoles/L (Total harness as CaCO3)

Test temperature:

20.2 to 20.5

pH:

7.7 to 7.8

Dissolved oxygen:

7.2 to 7.4

Salinity:

Not mentioned

Conductivity:

<5 µS/cm

Nominal and measured concentrations:

Nominal concentrations 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.27 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

There was no immobilization of daphnia in the negative control and at the lowest concentration of 0.4 mg/mL either at 24 or at 48 hours post-exposure. The immobilization of daphnia was 5, 20, 30, 50, 85, 95, and 100 % at 24 hours and 15, 45, 50, 70, 100, 100 and 100 % at 48 hours post-exposure at the tested concentrations of 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L of Clovanyl-3-Formate (CARYOLAN).

At 0.32 mg/L reference substance, the immobilization of daphnia was 40% and 55 % observed at 24 and 48 hours post exposure, respectively.

The EC50 value for Clovanyl-3-Formate (CARYOLAN) at 24 hours was 6.952 mg/L based on nominal concentrations with fiducial limits at 95 % being 5.0398 and 9.5909 mg/L.

The EC50 value for Clovanyl-3-Formate (CARYOLAN) at 48 hours was 3.27 mg/L based on nominal concentrations with fiducial limits at 95 % being 2.2667 and 4.7176 mg/L.

Results with reference substance (positive control):

At 0.32 mg/L reference substance, the immobilization of daphnia was 40% and 55 % observed at 24 and 48 hours post exposure, respectively.

Reported statistics and error estimates:

The EC50 value with 95% fiducial limits, and the equation for the dose-response curve was determined by Probit analysis by the method of Finney (1971) using developed and validated computer program.

Any other information on results incl. tables

TABLE 1.           Definitive Test – Results on Daphnia Immobilization

Group	Treatment (mg/L)	No. of Daphnia immobilized (5 Daphnia per replicate)								% Immobilization
		24 h				48 h				24 h	48 h
		R1	R2	R3	R4	R1	R2	R3	R4
G1	Negative control	0	0	0	0	0	0	0	0	0	0
G2	0.4	0	0	0	0	0	0	0	0	0	0
G3	0.88	1	0	0	0	1	1	1	0	5	15
G4	1.94	1	0	1	2	2	3	2	2	20	45
G5	4.27	1	1	2	2	2	2	4	2	30	50
G6	9.39	3	2	3	2	3	4	3	4	50	70
G7	20.66	4	4	5	4	5	5	5	5	85	100
G8	45.46	4	5	5	5	5	5	5	5	95	100
G9	100	5	5	5	5	5	5	5	5	100	100
G10	Reference substance 0.32 mg/L K2Cr2O7	2	3	1	2	3	3	3	2	40	55

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 value for Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate [Clovanyl-3-Formate (CARYOLAN)] at 48 hours was 3.27 mg/L based on nominal concentrations with fiducial limits at 95 % being 2.2667 and 4.7176 mg/L.

Executive summary:

The acute immobilization effect of the test item Clovanyl-3-Formate (CARYOLAN) was studied on Daphnia magna. The Daphnia magna of less than 24 hours old were exposed to concentrations of 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L of Clovanyl-3-Formate (Caryolan) along with a negative and reference substance in reconstituted water at 20 ± 1^oC. The number of daphnia immobilized was recorded at 24 and 48 hours post exposure.

There was no immobilization of daphnia in the negative control and at the lowest concentration of 0.4 mg/mL either at 24 or at 48 hours post-exposure. The immobilization of daphniawas 5, 20, 30, 50, 85, 95, and 100 % at 24 hours and 15, 45, 50, 70, 100, 100 and 100 % at 48 hours post-exposure at the tested concentrations of 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L of Clovanyl-3-Formate (CARYOLAN).

There was 40 and 55% immobilization of daphnia in thereference substance (potassium dichromate), at 24 and 48-hour post exposure, respectively, indicating the sensitivity of the test procedure used.

 

The EC₅₀value for Clovanyl-3-Formate (CARYOLAN) at 48 hours was 3.27 mg/L based on nominal concentrations with fiducial limits at 95 % being 2.2667 and 4.7176 mg/L.