Hydrocotyle asiatica, ext.

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From October 15 to October 17, 2019

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Test Item name: Centella asiatica dry extract
Batch No.: 972/43/A
Appearance: Dark green powder

Analytical monitoring:

yes

Vehicle:

yes

Remarks:

ISO medium

Test organisms (species):

Daphnia magna

Details on test organisms:

Twenty animals, divided into four replicates (glass beaker; volume app. 50 mL) of five animals each were exposed to the test concentration or run as control for 48 hours in a static test.
The immobility or mortality of the Daphnia was determined by visual observation after 24 and 48 hours of exposure.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

app. 249 mg/l (as CacCO3)

Test temperature:

The test temperature was in the range of 21.0 – 21.3°C measured in the test vessels during the test. The additionally measured ambient temperature within the climate chamber was between 20.3 – 21.7°C.

pH:

The pH of the test solutions was not adjusted and did not vary by more than 1.5 units in any one test. The pH was in the range of 7.63 – 7.88 during the test.

Dissolved oxygen:

The dissolved oxygen concentration was in the range of 7.26– 8.32 mg/L during the test.

Nominal and measured concentrations:

Based on the results of the preliminary test, a single test concentration at 100 mg/L nominal concentration and one control group were tested in a limit test. Biological results and endpoints are based on the nominal concentration.

Details on test conditions:

Light-Dark Cycle: The light-dark cycle during the test was 16 hours light (artificial illumination) and 8 hours darkness.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

In this 48-hour acute toxicity test on Daphnia magna, the obtained results showed that the test item Centella asiatica dry extract had no toxic effects on the mobility of the daphnids at the limit concentration of 100 mg/L.
All validity criteria were met. The results are based on the nominal test item concentration (100 mg/L) and were determined directly from the raw data.
The 48-h NOEC was determined to be 100 mg/L and the LOEC and the EC50 values were determined to be > 100 mg/L

Description of key information

The 48-h NOEC was determined to be 100 mg/L and the LOEC and the EC50 values were determined to be > 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The study was regarded as “ reliable with restriction” (Klimish score 2), because the toxicity values were determined on the basis of the analytical measurement of the main components Madecassicoside and of the Asiaticoside, that are expected to be hydrolytically instable (see hydrolysis study).