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EC number: 228-668-0 | CAS number: 6320-14-5
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- data is from peer-reviewed journal
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- List of colorants to be classified as toxic
- Author:
- R Anliker, G Diirig, D Steinle and E J Moriconi
- Year:
- 1 988
- Bibliographic source:
- Journal of the Society of Dyers and Colourists, Volume 104 May/June 1988,223-225
- Reference Type:
- review article or handbook
- Title:
- Ecological and toxicological properties of dyestuffs
- Author:
- Chittaradan Desai
- Year:
- 1 992
- Bibliographic source:
- Ecological and toxicological properties of dyestuffs, Chittaradan Desai,Colourage,December 1992,page No. 51-54
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Principles of method if other than guideline:
- Acute Oral toxicity test was carried out to study the effects of 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride (6320-14-5) on rats.
- GLP compliance:
- not specified
- Test type:
- other: no data available
- Limit test:
- no
Test material
- Reference substance name:
- 2-[3-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)prop-1-enyl]-1,3,3-trimethyl-3H-indolium chloride
- EC Number:
- 228-668-0
- EC Name:
- 2-[3-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)prop-1-enyl]-1,3,3-trimethyl-3H-indolium chloride
- Cas Number:
- 6320-14-5
- Molecular formula:
- C25H29ClN2
- IUPAC Name:
- 2-[3-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)prop-1-enyl]-1,3,3-trimethyl-3H-indolium chloride
- Test material form:
- other: Solid crystalline powder
- Details on test material:
- - Name of test material: 2-[3-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)prop-1-enyl]-1,3,3-trimethyl-3H-indolium chloride / Basic Red 12 / C.I. 48070
- IUPAC name: 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride
- Molecular formula: C25H29ClN2
- Molecular weight: 392.971 g/mole
- Smiles : c12c(C(C)(C)C(\N1C)=C/C=C/C=1C(c3ccccc3[N+]1C)(C)C)cccc2.[ClH-]
- Inchl: 1S/C25H29N2.ClH/c1-24(2)18-12-7-9-14-20(18)26(5)22(24)16-11-17-23-25(3,4)19-13-8-10-15-21(19)27(23)6;/h7-17H,1-6H3;1H/q+1;/p-1
- Substance type: Organic
- Physical state: Solid crystalline powder (Violet)
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report):C.I. Basic Red 12
- Molecular formula :C25H29N2.Cl
- Molecular weight :392.971 g/mol
- Substance type:organic
- Physical state:No data
- Purity :>98%
- Impurities (identity and concentrations):<2% (The purified test samples contained water and such inert ingredients as sodium chloride, sodium sulphate. etc.)
Test animals
- Species:
- rat
- Strain:
- not specified
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- No data available
Administration / exposure
- Route of administration:
- oral: unspecified
- Vehicle:
- not specified
- Details on oral exposure:
- No data available
- Doses:
- 25 mg/kg bw and 31 mg/kg bw
- No. of animals per sex per dose:
- No data available
- Control animals:
- not specified
- Details on study design:
- Details on study design
- Duration of observation period following administration: 14 days - Statistics:
- No data available
Results and discussion
- Preliminary study:
- No data available
Effect levelsopen allclose all
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 25 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 50% mortality was observed at dose 25 mg/kg bw
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 31 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 50% mortality was observed at dose 31 mg/kg bw
- Mortality:
- 50% mortality was observed at dose 25 mg/kg bw and 31 mg/kg bw
- Clinical signs:
- other: No data available
- Gross pathology:
- No data available
- Other findings:
- No data available
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 based on GHS criteria
- Conclusions:
- The lethal concentration (LD50) value for acute oral toxicity test was considered to be 25 mg/kg bw in female rats and 31 mg/kg bw in male rats ,when rats were treated with 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride (6320-14-5) orally according to OECD Guideline 401 (Acute Oral toxicity).
- Executive summary:
Acute oral toxicity study was performed in rats using test material 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride(6320-14-5).50% mortality was observed at dose 25 and 31 mg/kg bw. Hence,LD50 value was considered to be 25 mg/kg bw in female rats and 31 mg/kg bw in male rats,when rats were treated with 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride(6320-14-5) orally according to OECD Guideline 401 (Acute Oral toxicity).
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