5-sulphosalicylic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July - August 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

2008

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Version / remarks:

1996

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Four parallel samples were taken from the 100 mg/L concentration level and from the control sample.

Vehicle:

no

Details on test solutions:

The test solution used in the test was prepared by mechanical dispersion without using of any solubilising agent.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Waterflea
- Strain/clone: Straus
- Source: Laboratory of Hydrobiology (Central Agricultural Office, Directorate of Plant-, and Soil Protection) 2100 Gödöllő, Kotlán S. u. 3. Hungary
- Age at test start: Less than 24 hours old
- Feeding during test: no

ACCLIMATION
- Acclimation period: no, breeding conditions same as test conditions

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

249 mg/L (as CaCO3)

Test temperature:

20.0 – 20.2°C

pH:

5.55 – 7.97

Dissolved oxygen:

7.20 – 7.79 mg/L

Nominal and measured concentrations:

nominal concentration: 100 mg/L
measured concentration: 99 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers
- Material, fill volume: glass, 40 mL medium
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: ISO medium, according to OECD 202

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light (artificial illumination) and 8 hours darkness
- Light intensity: 609 lux

EFFECT PARAMETERS MEASURED
after 24 and 48 hours of exposure visual observation (immobility)

RANGE-FINDING STUDY
- Test concentrations: 100 mg/L nominal concentration
- Results used to determine the conditions for the definitive study: No immobility or any sub-lethal effect was detected in the test concentration and in the control group during the 48-h exposure period.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: measured EC50 for hydrat

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The measured EC50 is >100 mg/L, the calculated value for anhydrous substance is >85.8 mg/L
- Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no

Results with reference substance (positive control):

- Results with reference substance valid - yes
- Relevant effect levels: 24h EC50: 1.41 mg/L, (95 % confidence limits: 1.19 – 1.68 mg/L)

Validity criteria fulfilled:

yes

Conclusions:

The acute immobilisation of the test item to Daphnia magna was determined according to OECD guideline 202. The EC50 value after 48 h was determined to be > 100 mg/L (nominal).

Executive summary:

The acute immobilisation of the test item to Daphnia magna (STRAUS) was determined according to OECD Guideline 202 / EEC Directive 92/69/EEC Method C.2 (reference 6.1.3 -1). The study was conducted under static conditions over a duration of 48 hours in a limit test design. 20 test organisms were exposed to the test concentration and control. The test item solutions were clearly dissolved throughout exposure. The nominal test item concentration was 100 mg/L. A reference test is carried out with potassium dichromate as reference item at least twice a year and the reference toxicity determined. The EC50 value of the reference item at 1.41 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L. Water quality parameters of pH-value and dissolved oxygen concentration measured at 0 and 48 h were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled. The EC50 value and EC10 value for the test item were determined to be above 100 mg/L after 48 h, the calculated value for anhydrous for is >85.8 mg/L.

Description of key information

The acute immobilisation of the test item to Daphnia magna was determined according to OECD guideline 202. The EC50 value after 48 h was determined to be > 100 mg/L (reference 6.1.3 -1).

Additional information

The acute immobilisation of the test item to Daphnia magna (STRAUS) was determined according to OECD Guideline 202 / EEC Directive 92/69/EEC Method C.2 (reference 6.1.3 -1). The study was conducted under static conditions over a duration of 48 hours in a limit test design. 20 test organisms were exposed to the test concentration and control. The test item solutions were clearly dissolved throughout exposure. The nominal test item concentration was 100 mg/L. A reference test is carried out with potassium dichromate as reference item at least twice a year and the reference toxicity determined. The EC50 value of the reference item at 1.41 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L. Water quality parameters of pH-value and dissolved oxygen concentration measured at 0 and 48 h were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.

The EC50 value and EC10 value for the test item were determined to be above 100 mg/L after 48 h, the calculated value for anhydrous for is >85.8 mg/L.