Reaction mass of isopropyl dihydrogen phosphate and phosphoric acid and propan-2-ol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

Feb 1966

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Study design similar to OECD Guideline with some deviations; summary report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1966

Report date:

1966

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

only dilutions tested

GLP compliance:

no

Remarks:

performed before GLP

Test material

Test material information

Constituent 1

Reference substance name:

(propan-2-yloxy)phosphonic acid; phosphoric acid; propan-2-ol

EC Number:

908-998-8

Molecular formula:

can not be determined for reaction masses

IUPAC Name:

(propan-2-yloxy)phosphonic acid; phosphoric acid; propan-2-ol

Test animals / tissue source

Species:

rabbit

Strain:

other: Yellow-silver strain

Details on test animals or tissues and environmental conditions:

TEST ANIMAL
- Housing: individually
- Diet (e.g. ad libitum): Standard Altromin K (Altrogge, Lage/Lippe, Germany), ad libitum
- Water (e.g. ad libitum): tap water, ad libitum

Test system

Vehicle:

physiological saline

Controls:

not required

Amount / concentration applied:

0.1 mL of 1:10 or 1:100 dilution

Observation period (in vivo):

1 hour post application
3 hour post application
7 hour post application
24 hours post application
48 hours post application

Number of animals or in vitro replicates:

3

Details on study design:

After treatment the eyes were not washed out.

Results and discussion

In vivo

Irritant / corrosive response data:

- 1:10 dilution:
eyes were slightly reddened, slight to moderate opacity of the cornea was observed, conjunctivae were swollen

- 1:100 dilution:
no effect

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye) based on GHS criteria

Conclusions:

The application of a 1:10 dilution of the test substance to the eyes of 3 rabbits lead to slight to moderate opacity of the cornea, reddening and swelling of the conjunctivae.
Therefore it is concluded that the undiluted registration substance has to be classified as irritant to the eyes, Cat. 1 (H318 - Risk of serious damage to the eyes; H318 - Causes serious eye damage) according to the criteria laid down in the EU Classification Labelling and Packaging Regulation (1272/2008/EC).

Executive summary:

A 1:10 and 1:100 dilution of the registrationsubstance were tested for its eye irritant properties in 3 rabbits.The study was performed similar to OECD Guideline 405.

The application of a 1:10 dilution of the test substance to the eyes of 3 rabbits lead to slight to moderate opacity of the cornea, reddening and swelling of the conjunctivae.

Therefore it is concluded that the undiluted registration substance has to be classified as irritant to the eyes (H318 - Causes serious eye damage) according to the criteria laid down in the EU Classification Labelling and Packaging Regulation (1272/2008/EC).