Tetrasodium 2-[[8-[[3-[(5-chloro-2,6-difluoro-4-pyrimidinyl)amino]benzoyl]amino]-1-hydroxy-3,6-disulphonato-2-naphthyl]azo]naphthalene-1,5-disulphonate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June from 25 to 29, 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Reason / purpose for cross-reference:

reference to other study

Remarks:

validated analytical method

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

yes

Remarks:

not impacting the validity and acceptance of the test (details are given below)

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Water solubility:

144.59 g/L

Conc. based on:

test mat.

Incubation duration:

24 - 72 h

Temp.:

20 °C

pH:

7.6 - 7.7

Details on results:

The water solubility ofthe test item was determined to be 144.59 ± 9.05 g/l (95 % confidence interval) at 20 ± 0.5 °C and pH 7.6 -7.7.
The repeatability of the determination was < 15 % and thus within the range required by the guidelines.

Sampling time	Measured concentration of test item, g/l		Measured pH
	Test vessels	Mean
after 24 hours	148.81	147.8	7.7
	148.53		7.7
	146.07		7.7
after 48 hours	143.27	145.35	7.6
	139.09		7.6
	153.68		7.6
after 72 hours	128.8	140.63	7.6
	136.55		7.6
	156.55		7.6
Mean with confidence interval (95 %)	-	144.59 ± 9.05	-
RSD %	-	3	-

Conclusions:

144.59 g/l at 20 °C and pH 7.6 -7.7

Executive summary:

The water solubility of the test item was determined in accordante with the method and procedures outlined into the OECD guideline 105.

A preliminary test for solubilitywas carried out for the decision whether the column elution method or the flask method has to be used for the performance of the main test. The solubility of the test item was higher than 10 mg/l, therefore, the flask method was used for the main test. The concentration in the clear aqueous phase was determined by HPLC-UV/Vis validate method.

About 38 g test item was weighed into stoppered Erlenmeyer flasks; after the addition of 50 ml ultrapure water, the flasks were agitated at 30 °C. After one day, three of the vessels were removed and re-equilibrated at 20 °C for 24 hours. The content of the vessel was then centrifuged at 20 °C and the concentration in the clear aqueous phase was determined. The other flasks were treated similarly after initial equilibration at 30 °C for two and three days, respectively. The samples were diluted before the analysis (two parallel dilutions). The pH of each sample was recorded.

The water solubility ofthe test item was determined to be 144.59 ± 9.05 g/l (95 % confidence interval) at 20 ± 0.5 °C and pH 7.6 -7.7.

The repeatability of the determination was < 15 % and thus within the range required by the guidelines.

Conclusion

144.59 g/l at 20 °C and pH 7.6 -7.7

Description of key information

144.59 g/l at 20 °C and pH 7.6 -7.7

Key value for chemical safety assessment

Water solubility:

144.59 g/L

at the temperature of:

20 °C

Additional information

OECD 105 - Flask method